摘要
目的:制备载乳铁蛋白壳聚糖微球/nHA/Co复合材料作为组织工程材料拟修复骨缺损。方法:通过乳化交联法法制备载乳铁蛋白壳聚糖微球,化学沉淀法制备纳米羟基磷灰石,改良酸酶法提取Ⅰ型胶原,应用京尼平交联制备复合材料,SEM检测材料的微观结构,测定壳聚糖微球对乳铁蛋白的载药率和包封率。结果:复合材料及各组成成份扫描电镜观察均具有较好的微观形态,壳聚糖微球对乳铁蛋白的载药率为(1.06±0.06)%,包封率为(88.2±2.9)%。结论:载乳铁蛋白壳聚糖微球/nHA/Co复合材料具有较好的微观形态。
Objective： To prepare composite material with lactoferrin-loaded chitosan microspheres/nHA/Co as tissue engineering material intending to repair bone defects. Methods： Recombinant human lactoferrin was loaded in to chitosan microspheres by emulsion crosslinking method. Nanosized hydroxyapatite was prepared through chemi- cal precipitation. Collagen-Ⅰwas extracted by acid dissolution and protease digestion. The composite materials was cross-linked by genipin, detected by SEM and determinated the loading rate and entrapment efficiency of lactoferrin. Results： All the composition and the composite material formed fine micromorphology. The loading rate and entrapment efficiency of lactoferrin was 1.06±0.06% and 88.2±2.9%. Conclusion： The composite material nHA/ Co with lactoferrin-loaded chitosan microsvheres formed good microstrueture.
出处
《口腔医学研究》
CAS
CSCD
2012年第3期195-197,201,共4页
Journal of Oral Science Research
基金
吉林省科技厅资助项目(编号:201115107)
吉林省科技厅资助项目(编号:20090437)
关键词
乳铁蛋白
壳聚糖微球组织工程骨缺损
Lactoferrin Chitosan microspheres Bone tissue-engineering Bone defect
