摘要
实验采用乳化交联法,使用复合交联剂(先用甲醛交联,再用戊二醛交联),制得盐酸四环素壳聚糖缓释微球,并考察不同分子量的壳聚糖、原料质量比、交联剂用量、复合交联剂用量、搅拌速度对微球的影响,筛选出最佳条件制备出载药微球,并研究了该微球在扫描电镜和倒置式研究型显微镜下的形态及其在pH=7.4,温度为37℃时的释放规律。结果表明,采用复合交联剂的乳化交联法所制得的微球球形好,粒径分布为5～50μm之间,载药量为26.9%,包封率为56.3%,并且具有良好的缓释效果。
The experimentation used composite crosslinker （formaldehyde and glutaraldehyde） to prepare tetracycline hydrochloridel chitosan sustained-release microspheres by emulsion cross-linking method. In order to select the optimum technology for preparing chitosan sustained-release microspheres, the influencing factors of drugcarried mierospheres were analysed,including the types of chitosan, the mass ratio of raw materials, the dosage of crosslinking agent and the emulsifying speeds. Moreover,the experimentation researched the modality of microspheres by scanning electron microscope and chatelier-type microscope. And the in-vitro release characteristics of microspheres were investigated under the conditions of pH = 7.4 and temperature is 37℃. The results suggest that prepared with emulsion cross-linking method in this test, the sphericity of microspheres was good, the diameter of microspheres was from 5-50μm. The drugloading was 26. 9%, and the enbedding ratio was 56.3%, and the in vitro release of tetracycline hydrochloridel from the microspheres is sustained.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2009年第6期959-961,965,共4页
Journal of Functional Materials
基金
国家自然科学基金资助项目(50573061)
四川省应用基础研究资助项目(07JY029-065)
西南交通大学科学研究基金资助项目(2006B52)
关键词
壳聚糖微球
盐酸四环索
复合交联
缓释性能
chitosan microspheres
tetracycline hydrochloride
compound cross-linked
sustained-release properties
