摘要
目的:探讨单次15Gy60Coγ射线照射对小型猪腮腺细胞凋亡与增殖的影响以及肿节风对其损伤的防护作用。方法:45只小型猪随机分成空白组、单照组与药照组3个大组,每组分成a、b、c三个平行组。药照组在照射前1周开始给药,空白组与单照组给予等量生理盐水。照射组在麻醉条件下给予15Gy60Coγ射线照射,空白组同等麻醉下予0Gyγ射线照射。a、b、c三个平行组分别于照射后第10天、40天、90天取双侧腮腺,以TUNEL法检测细胞凋亡,以免疫组织化学法检测细胞PCNA的表达。结果:单照组与药照组在各时间点腮腺细胞凋亡率均高于空白组,与空白组相比差异均有统计学意义(P≤0.01);第10天,药照组凋亡率低于单照组,差异有统计学意义(P≤0.01)。单照组与药照组PCNA表达率在各时点均高于空白组,与空白组相比差异均有统计学意义(P≤0.01);而各时点比较,药照组PCNA表达率均高于单照组,差异有统计学意义(P≤0.01)。结论:电离辐射可能通过诱导腮腺细胞凋亡而致使腮腺受损,同时机体可能通过增殖代偿以修复损伤。而使用肿节风有助于降低凋亡率,促进增殖修复。
Objective To investigate the effect of a single dose of 15 Gy gamma-rays on apoptosis and proliferation of parotid gland ceils in the miniature pig and the potential protective effect of glabrous sarcandra herb against radiation injury. Methods Forty-five miniature pigs were randomized into normal control, radiation and radiation plus medicine-treated groups, then each group was randomized into three parallel group which were named a, b and c. Glabrous sarcandra herb was administered to the miniature pigs in the radiation plus medicine-treated group a week before exposure to radiation, and the equivalent does of physiological saline was administered to miniature pigs in other two groups. Miniature pigs in the radiation group and the radiation plus medicine-treated group received a single dose of 15 Gy gamma-rays delivered to each parotid gland, miniature pigs in the normal control group received 0 Gy gamma-rays. On the 10th day, 40th day and 90th day after radiation, the parotid glands of miniature pigs were collected under anesthesia, and the parotid gland cell apoptosis was detected by TUNEL assay, PCNA expression was detected by immunohistochemistry staining technique. Results The parotid gland cell apoptosis rate in the radiation group and the radiation plus medicine-treated group was higher than that in the normal control group at every time point with significant difference （P 〈 0.01）. the parotid gland cell apoptosis rate in the radiation plus medicine-treated group was lower than that in the radiation group on the 10th day with significant difference （P 〈 0.01）. PCNA expression in the radiation group and the radiation plus medicine-treated group was higher than that in the normal control group at every time point with significant difference （P 〈 0.01）, and PCNA expression in the radiation plus medicine-treated group was higher than that in the radiation group at every time point with significant difference （P 〈 0.01）. Conclutlon Ionizing radiation may damage the parotid gland by inducing the parotid gland cell apoptosis, and this injury can be restored by parotid gland cell proliferation. The protective effect of the glabrous sarcandra herb was attributed to reducing the parotid gland cell apoptosis and promoting cell proliferation.
出处
《实用医学杂志》
CAS
北大核心
2012年第6期885-888,共4页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:30960103)
关键词
肿节风
小型猪
腮腺
凋亡
增殖
Glabrous sarcandra herb
Miniature pig
Parotid gland
Apoptosis
Proliferation
