摘要
目的:观察兔慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)模型鼻窦黏膜上皮核因子-κB(NF-κB)p65蛋白表达的变化及鼻渊舒口服液对其的影响,探索鼻渊舒治疗CRS的可能机制。方法:取新西兰大白兔100只,适应性喂养1周后,随机分为正常组、模型组、假手术组、鼻渊舒组、西药治疗组,每组20只,建立CRS模型;鼻渊舒组、西药治疗组分别给予鼻渊舒口服液ig 4.05 g.kg-1,克拉霉素ig 25 mg.kg-1,共14 d,处死后取鼻窦黏膜组织HE染色光镜观察鼻窦黏膜病理变化,Westernblotting法检测鼻窦黏膜上皮细胞胞质及细胞核NF-κB p65蛋白表达。结果:模型组鼻窦黏膜呈慢性炎症表现,黏膜炎细胞浸润,上皮细胞、腺体和杯状细胞明显增生;胞质及细胞核NF-κB p65蛋白表达较正常组显著增高(P<0.01)。经鼻渊舒治疗后鼻窦黏膜上皮修复较好,炎细胞浸润、腺体和杯状细胞增生不明显;胞浆NF-κB p65蛋白表达虽显著低于模型组(P<0.01),但显著高于正常、假手术及西药组(P<0.01);胞核NF-κB p65蛋白表达显著低于模型组(P<0.01),与正常、假手术及西药组比较无显著性差异。结论:NF-κB参与了CRS的发生,鼻渊舒口服液主要通过抑制NF-κB p65向细胞核易位而起治疗作用。
Objective： To investigate the influence of Biyuanshu （BYS） nuclear factor-kappa B （NF-κB） p65 expresSion of nasal sinuses mucosa epithelial in rabbit chronic rhinosinusitis （CRS） model, and explore its possible molecular mechanism. Method： One hundred New Zealand rabbits were randomly divided into normal group, model group＇ sham operation group, BYS group, western medicine group, with 20 in each group, and CRS model was established. BYS group was given BYS 4.05 g . kg-1. d-1, western medicine group given clarithromycin 25 mg . kg-1.d-1 for 14 days. Nasal sinuses mucosa tissue was collected to observe nasal sinuses mucosa pathological changes with light microscopy after HE dyeing, and detect nasal sinuses mucosal epithelium cytoplasm and nucleus NF-KB p65 protein expression with Western Blotting. Result： Model group appeared chronic inflammation and inflammatory cell infiltration, epithelial cells a/td glandular organs and goblet cells were hyperplasia obviously. Nasal sinuses mucosal epithelium cytoplasm and nucleus NF-κB p65 protein expression was higher than normal group （P 〈 0.01 ）. After the treatment with BYS, the nasal sinuses mucosa epithele wasimproved, inflammatory cells and glandular organs and goblet cells were not hyperplasia obviously. Nasal sinuses mucosal epithelium cytoplasm NF-κB p65 protein expression was higher than normal group and sham operation group and western medicine group obviously （P 〈0. 01 ） , nasal sinuses mucosal epithelium nucleus NF-KB p65 protein expression was lower than model group obviously （P 〈0.01）, but there were no differences among nomal group and sham operation group and western medicine group, Conclusion： NF-κB may take part in the development of CRS, BYS and suppress transposing of NF-κB p65 to cell nucleus.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期151-154,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30801480
81102621)
成都中医药大学科技发展基金项目(ZRYB200945)
