摘要
为探讨孕期饮酒对胚胎生长发育的影响,应用植入后全胚胎培养(w hole em bryo culture, WEC)进行研究。将孕9.5天龄鼠胚胎移植于酒精终浓度分别为0、0.40、1.00、2.00、4.00g/L的全胚胎培养基中,于体外培养48小时,观察酒精对胚胎发育和器官形态分化影响。结果表明酒精对胚胎发育具有明显影响且呈剂量-效应关系,0.40g/L组胚胎生长发育指标和器官形态分化指标与对照组差异无显著性(P> 0.05) ,1.00g/L组显著影响中脑、前脑、神经管、卵黄囊循环和翻转等指标,2.00g/L以上组各指标显著低于对照组(P< 0.05) ,胚胎致畸率和死亡率显著增高。结论认为酒精具有发育毒性和致畸性,酒精作用的主要靶器官是脑。
In order to explore the effects of drinking alcohol during pregnancy on embryonic development and its mechanism, a post implantation whole embryo culture(WEC) technique was used. The 9 5 day rat embryos were explanted in rat serum medium(immediately centrifugal serum,ICS) with alcohol(0,0 4,1 0,2 00 and 4 00g/L), and cultured for 48 hours. The index of embryo development and morphological scores induced by alcohol were observed. The result showed that alcohol had obviously effects on the development and growth of embryos with a dose response relationship. Embryonic development of 0 4g/L group was not significantly different from the control group, whereas 1 0g/L group could interfere with the development score of mid brain, forebrain, neurotube, and visceral yolk sac(VYS) circle obviously. All scores of the 2 00g/L group were significantly lower than that of control group(P<0 05). Moreover, the rate of embryo lethality and teratogenecity were obvious increased. It is concluded that alcohol has developmental toxicity and teratogenicity. The target organ affected by alcohol is brain. The effects of alcohol on the developmental differentiation of visceral yolk sac and DNA synthesis are probably related to its developmental abnormalities.
出处
《卫生研究》
CAS
CSCD
北大核心
2000年第1期17-19,共3页
Journal of Hygiene Research
基金
中国博士后基金!资助项目(No.98- 09)
四川省计划生育委员会项目!(No.98- 007)
关键词
植入后全胚胎
培养
酒精
胚胎发育
post implantation whole embryo culture, alcohol, embryo development
