摘要
用体外全胚胎培养模型并结合电镜技术等探讨了硒、砷对卵黄囊及胚胎的影响。结果显示。随着硒、砷剂量的增高,对卵黄囊及胚胎的损害逐渐增强,呈明显的剂量-反应关系。>1.0mg/L硒和>0.5mg/L砷均可诱发卵黄囊生长和血管分化不良、畸胎发生率增高等。组织学检查发现卵黄囊厚度变薄,内皮层细胞表面微绒毛数量减少、变短和结构紊乱;细胞内溶酶体、线粒体、内质网的数量和结构亦有明显改变。间质层血岛、微血管和红细胞的数量也明显减少。在硒、砷联合染毒组,3×4析因分析结果表明,二者交互作用的主效应呈拮抗作用(P<0.001),卵黄囊的大体、细胞和亚细胞结构恢复正常,畸胎率也明显降低。结果提示卵黄囊结构和功能的改变与硒、砷的致畸机制密切相关,二者能在卵黄囊水平上相互缓解其发育毒性。
The effects of single and combined toxicity of selenium(Se^(+4)) and arsenic(As^(+3)) on the development of Sprague-Dawley rat yolk sac and embryo were studied during early organogenesis (head-fold stage) using whole embryo culture technique and electron microscopy. The results showed that selenium and arsenic would damage visceral yold sac and embryos in dose-respose relationship. At the concentrations of >1. 0mg/L Se^(+4) and >0.5mg/L As^(+3), the retardation of yolk sac growth and circulatory establishment, and the rise of incidence of abnormal embryos were noticed. It was observed that the numbers of yolk-sac vessels, blood islands, red blood cells, microvilli, lysosomes, mitochondria and endoplasmic reticula decreased and relevant uhrastructures were damaged in dose related manner. By means of factorial experiment and analysis (3×4), it has been discovered that the main effect of interaction between selenium and arsenic showed antagonism (P=0.0001). No abnormal embryos came into view. Morphological structrues of yolk sac were not different from those of the control group in 0.5mg/L Se^(+4)+1.0mg/L As^(+3), 2.0mg/L Se^(+4)+0.5mg/L As^(+3) and 2.0mg/LSe^(+4)+1.0mg/L As^(+3) groups. Our investigations suggested that the changes of yolk-sac structure and nutrient function would be related to teratogenie machanism of selenium and arsenic.
出处
《卫生研究》
CAS
CSCD
北大核心
1996年第1期16-19,共4页
Journal of Hygiene Research
基金
国家自然科学基金
关键词
卵黄囊
全胚胎培养
硒
砷
畸胎学
毒性
selenium
arsenic
yolk-sac
whole embryo culture
combined action
