摘要
目的了解长春地区四种主要腹泻病毒病原构成及流行病学特点。方法收集长春市儿童医院5岁以下住院患儿腹泻样本共460例，轮状病毒采用ELISA试剂盒检测，杯状病毒、星状病毒采用逆转录一聚合酶链反应（RT-PCR）法，腺病毒采用聚合酶链反应（PCR）法进行鉴定。结果460份标本中轮状病毒占35．22％（162／460）；杯状病毒占20．43％（94／460），星状病毒占9．78％（45／460），腺病毒占3．70％（17／460），混合感染达7．17％（33／460），发病患儿以2岁以下婴幼儿为主。对162份轮状病毒阳性标本进行G／P分型，结果示G1P[8]为主要流行株，杯状病毒以GII-4亚型为主要流行株，星状病毒为I型，腺病毒为Ad41。结论长春地区婴幼儿病毒性腹泻的病原中轮状病毒是最主要病原，其次为杯状病毒、星状病毒和腺病毒。
Objective To study the pathogen and characteristics of viral diarrhea in children in Changchun area. Methods 460 stools specimens were collected from children with acute diarrhea cured in the childrens,hospital of Changchun in 2010. Rotavirus were detected by ELISA, caliceverus and astrovirus were detected by reverse transcription-polymerase chain reactions （RT-PCR） , adenovirus were detected by polymerase chain reactions （PCR）. Results A total of 460 specimens were detected. The detection rate of rotavirus, caliceverus, astrovious, adenovious respectively is 35.22% , 20.43% , 9.78% , 3.70% , the detectablerate of mixed infection is 7.61% , children under 2 years old were the major patient. The main genotypes of the virus ： rotavirus （ G3P [ 8 ] ） , caliceverus （ GII-4 ） , astrovious （ typeI ）, adenovious （ Ad41 ）. Conclusion Rotavirus is the main pathogen in Changchun. Followed by caliceverus,astrovious,adenovious.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2012年第1期5-7,共3页
Chinese Journal of Experimental and Clinical Virology
