摘要
利用3个优化的STS标记对陕西省1960年以来引进和自育的99份小麦品种(系)的Waxy蛋白基因的变异情况进行了鉴定。结果表明,Wx-A1位点的标记在Wx-A1a和Wx-A1b基因型内分别扩增出336 bp和317 bp特异片段;Wx-B1a基因型内扩增出425 bp特异产物,Wx-B1b基因型内不扩增出此产物;Wx-D1a和Wx-D1b基因型内分别扩增出867 bp和279 bp特异产物。检测出除陕糯1号基因型为Wx-A1b+Wx-B1b+Wx-D1b外,Wx-B1b基因缺失突变材料12份,无Wx-A1b基因和Wx-D1b基因缺失突变材料。这3个标记可以用于分子标记辅助育种,并提供了部分品种的缺失类型。
Three optimized STS markers were used to analyze 99 wheat cultivars in Shaanxi from 1960 with different types of Waxy proteins.The results indicated that the marker on Wx-A1 locus amplified 336 bp and 317 bp special fragments associated to Wx-A1a and Wx-A1b genotypes;the marker on Wx-B1 amplified 425 bp special fragment associated to Wx-B1a and null special fragment associated to Wx-A1b genotype and the marker on Wx-D1 amplified 867 bp and 279 bp special fragments associated to Wx-D1a and Wx-D1b genotypes,respectively.Except for the cultivar Shaannuo 1 with the genotype of Wx-A1b ＋ Wx-B1b ＋ Wx-D1b,12 cultivars were identified to null Wx-B1 mutants and no cultivars were detected for null Wx-A1 mutants and null Wx-D1 mutants.The three optimized STS markers were demonstrated to be useful tools to identify wheat cultivars with mutant and normal alleles of the Waxy genes in marker-assisted selection of wheat breeding programs and the genotypes of Waxy genes of some materials were released in this paper.
出处
《华北农学报》
CSCD
北大核心
2011年第6期67-72,共6页
Acta Agriculturae Boreali-Sinica
基金
陕西省教育厅项目(2008JK212)
安康学院科技创新团队项目(2008AKXY006)
关键词
普通小麦
地方品种
Waxy蛋白基因
PCR标记
多样性
Bread wheat（Triticum aestivum L.）
Landrace
Waxy protein gene
Molecular marker
Diversity
