摘要
为了探讨小麦茎节伸长的分子机理,在DDRT-PCR技术分析小麦拔节过程中的基因差异表达谱基础上,克隆了一个在拔节过程中沉默表达的基因,命名为WSR1。对该基因cDNA片段的序列和生物信息学分析显示,WSR1为Ser/Thr蛋白激酶基因,其编码蛋白与大麦中编码Ser/Thr蛋白激酶的序列具有68%的同源性。通过半定量RT-PCR技术分析显示,该基因在小麦拔节过程中的表达模式与通过DD-RT-PCR分析所展示的差异表达趋势基本上一致。
To study the molecular mechanism of wheat stem elongation, the differential display reverse transcription PCR （DDRT-PCR） was used to identify the gene expression pattern during the basal stem elongation in wheat plant. On the basis of gene different expression profiles, one cDNA which observed in pre-elongation basal stem tissue, but not in elongated was cloned, named WSR1. Sequence analysis and homology search in GenBank database showed that WSR1 had 68% similarity to barley serine/threonine protein kinase; The expression patterns of the cloned cDNAs were analyzed via semi-quantitative RT-PCR in the elongating wheat stem, the results showed that the expression patterns was basically agree with the expression trends displayed in DDRT-PCR analysis.
出处
《山西农业科学》
2009年第3期22-24,共3页
Journal of Shanxi Agricultural Sciences
基金
山西省国际科技合作项目(2008081007)
太原市技术创新计划项目(0801009)
山西省农业科学院青年基金项目(YQN0602)
关键词
小麦
茎节伸长
基因差异表达
Wheat
Stem elongation
Gene differential expression
