摘要
为加快谷子杂种优势利用的研究进展、寻找谷子光敏雄性不育基因,利用SSR方法对谷子光敏雄性不育基因进行了分子标记。首先用166对引物在谷子光敏不育系GM与恢复系恢东1号两亲本间进行了筛选,其中有61对引物在亲本间存在差异;经F2群体153株单株验证后,仅有一对引物b159和目标基因连锁;通过Kosambi函数计算,其连锁距离为13.5 cM,位于第6条染色体。
To accelerate the research progress of heterosis utilization in millet and to look for photo-sensitive male sterility genes, millet photo-sensitive male sterile gene was studied using SSR technique. 166 pairs of SSR primers were selected between photo-sensitive male sterile material GM and restorer material HuiDong 1.61 pairs primers were different between their parents. Checking by F2 population 153 strains only b159 was the linked marker of target gene. The chain distance is 13.5 cM through Kosambi function. This mark is located at the sixth chromosome
出处
《华北农学报》
CSCD
北大核心
2011年第5期112-116,共5页
Acta Agriculturae Boreali-Sinica
基金
山西省自然科学基金项目(2008011059-3)
关键词
谷子
光敏雄性不育基因
SSR
分子标记
Millet
Photo-sensitive male sterile gene
SSR
Molecular marker
