摘要
目的:考察不同炮制方法及炮制前后吴茱萸中吴茱萸碱、吴茱萸次碱和柠檬苦素的含量变化情况。方法:采用高效液相色谱法,Hypersil ODS C_(18)(250 mm×4.6 mm,5μm);流动相:乙腈-水-四氢呋喃-乙酸(41:59:1:0.2);流速:1.0 ml·min^(-1);检测波长:225 nm;柱温:25℃。结果:吴茱萸碱、吴茱萸次碱、柠檬苦素进样量分别在0.156～3.120μg(r=0.999 6,n=6)、0.116～2.320μg(r=1.000 0,n=6)、0.620～12.400μg(r=0.999 1,n=6)范围内与峰面积呈良好线性关系,平均回收率分别为97.2%(RSD=1.33%)、97.1%(RSD=1.56%)、97.3%(RSD=1.52%)。结论:不同炮制方法吴茱萸碱、吴茱萸次碱、柠檬苦素含量不同,砂烫法优于其他炮制方法。
Objective： To determine the contents of evodiamine,rutaecarpine and obaculactone in the different processed products of Evodia r,ttaecarpa（ Juss. ）Benth. Method： A Hypersil C ls （250 mm × 4. 6 mm,5 μm） was used. The mobile phase was acetonitrile- water-tetrahydrofuran-acetic acid （41 ： 59：1 ： 0. 2） with the flow rate of 1：0 ml. min-1. The temperature was 25 ℃ and the detection wavelength was 283 nm. Result： The calibration curves were linear within the range of 0. 156-3. 120 for evodiamine （r = 0. 999 6, n = 6 ） ,0. 116-2. 320 for rutaecarpine （r = 1. 000 0, n = 6） and 0. 620-12. 400 for obaculactone （r = 0. 999 1, n = 6）. The average recovery of evodiamine,rutaecarpine and obaculactone was 97. 2% with RSD of 1.33% ,97. 1% with RSD of 1.56% and 97.3% with RSD of 1.52% ,respectively. Conclusion： Different processing methods of Evodia rutaecarpa（Juss. ） Benth can influence the contents of evodiamine, rutaecarpine and obaculactone. The sand-scorch method is superior to the other ones.
出处
《中国药师》
CAS
2011年第12期1742-1744,共3页
China Pharmacist
基金
湖南省中医药管理局课题(编号:2009032)
