摘要
目的:建立紫外分光光度法测定天麻中微量元素硒的方法。方法:采用微波消解技术处理样品,加入HCl将Se6+还原成Se4+,Se4+与邻苯二胺络合,用甲苯萃取,紫外分光光度法测定天麻中的微量元素硒。结果:相关系数r=0.9994,加标回收率为96.6%～103.7%,最大吸收波长为348 nm,硒含量在0.01μg/kg～8.00μg/kg范围内符合比尔定律。结论:该方法简单快速、灵敏度高、准确度好和检出费用低等优点,适合基层实验室对食品中微量元素硒的测定。
Objective： to establish a method for determination of selenium content in gastrodia by ultraviolet spectrophotometry.Methods： the use of microwave digestion technique for processing samples,addition of HCl,in which the Se6＋ was reducted to Se4＋ and tetravalent selenium complexed with o-Phenylenediamine,extraction with toluene,using ultraviolet spectrophotometric method to determine selenium in gastrodia.Results： the correlation coefficient r=0.9994,the recovery of standard addition is 96.6%～103.7%,wavelength Max.absorbance is 348 nm,selenium content in the 0.01 μg/kg～8.0 μg/kg range meets Beer law.Conclusion： the experimental results show the method is simple,rapid,high sensitivity,the result is accurate,the test cost is low,and it is easy for the determination of selenium in the basic unit laboratory.
出处
《云南化工》
CAS
2011年第6期36-38,共3页
Yunnan Chemical Technology
关键词
紫外分光光度法
邻苯二胺
天麻
硒
微波消解
Ultraviolet spectmphotometry
o-Phenylenediamine
Gastmdia
Selenium
microware digestion
