摘要
目的 探讨血小板衍生生长因子 (PDGF AB)促进伤口愈合的作用机理及其在瘢痕增生中的作用。方法 取体外培养的人正常皮肤及增生性瘢痕成纤维细胞 ,用原位杂交法观察PDGF对两种细胞表达Proα1(Ⅲ )mRNA的影响。结果 PDGF AB对两种成纤维细胞表达Proα1(Ⅲ )mRNA均有明显促进作用 ,且均呈剂量依赖性关系 ,但作用有差异。结论 PDGF AB可能通过刺激成纤维细胞合成胶原等细胞外基质促进伤口愈合 ,同时也可导致胶原等细胞外基质的沉积 ,从而促进瘢痕增生。
Objective To investigate the mechanism of PDGF AB to promote wound healing and its role during the process of scar formation.Methods In vitro cultured fibroblasts of human normal skin and hypertrophic scar were treated with PDGF AB.The expression of Pro α 1(Ⅲ) mRNA of the fibroblasts was evaluated by in situ hybridization technique.Results PDGF AB could increase Pro α 1(Ⅲ)mRNA expression of fibroblasts of both normal skin and hypertrophic scar in a dose dependent manner.However,the effect of PDGF AB on hypertrophic scar fibroblasts was stronger.Conclusions PDGF AB may promote wound healing by stimulating extracelluar matrix synthesis,e.g. collagen.PDGF AB may also play a role in scar formation through collagen deposition.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2000年第2期78-80,共3页
Chinese Journal of Plastic Surgery
