摘要
目的：探讨ＰＤＧＦ－ ＡＢ促进伤口愈合及其在瘢痕增生中的作用机理。方法：取体外培养的人正常皮肤及增生性瘢痕成纤维细胞（ｎｏｒｍａｌｓｋｉｎｆｉｂｒｏｂｌａｓｔ，ＮｓＦｂ，ｈｙｐｅｒｔｒｏｐｈｉｃ ｓｃａｒｆｉｂｒｏｂｌａｓｔ，ＨＴｓＦｂ） ，用ＭＴＴ和３Ｈ－ ＴｄＲ掺入法观察ＰＤＧＦ－ ＡＢ对两种细胞增殖及ＤＮＡ合成的影响。结果：ＰＤＧＦ－ ＡＢ对两种成纤维细胞增殖及ＤＮＡ合成均有明显刺激作用，且均呈剂量依赖性关系，但作用有差异。结论：ＰＤＧＦ－ ＡＢ可能通过刺激成纤维细胞增殖及ＤＮＡ合成促进伤口愈合，但同时在瘢痕增生性疾病中可能起重要促进作用。
Purpose To investigate the mechanism by which PDGF-AB promotes wound healing and the effect of PDGF-AB on scar forming.Methods:In vitro cultured human normal skin and hypertrophic scar fibroblasts were applied to measure the effect of PDGF-AB on proliferation and DNA synthesis of fibroblasts by MTT and 3 H-TdR incorporation.Results:There was difference of proliferation and DNA synthesis between human normal skin and hypertrophic scar fibroblasts,PDGF-AB could enhance proliferation and DNA synthesis of two kinds of fibroblasts in dependent dose.Conclusion:PDGF-AB may promote wound healing by stimulating proliferation and DNA synthesis of fibroblasts,and may play important role in scar forming.
出处
《西北国防医学杂志》
CAS
1999年第4期274-276,共3页
Medical Journal of National Defending Forces in Northwest China
