摘要
本文观察了不同浓度的铜、锌对培养乳鼠心肌细胞缺氧/再给氧损伤后超氧歧化酶(superoxid dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-px)活性和脂质过氧化(lipid peroxidation,LPO)代谢产物丙二醛(malondialodehyde,MDA)含量改变,以及乳酸脱氢酶(lactate dehydrogenase,LDH)释放的影响。结果表明:缺氧/再给氧后,心肌细胞SOD活性降低,GSH-px活性和MDA含量增高,LDH释放增加。铜、锌均能不同程度地提高SOD活性,但铜的作用强于锌。锌、铜均可降低LDH的释放,但锌的作用强于铜,锌、铜浓度过高反而增加LDH的释放。此外,铜使GSH-px活性明显升高,并显著降低MDA含量。
The effect of various concentrations of copper and zinc on anoxia-reoxygenation in the neonatal rat heart cell cultures was studied by determining the activity of superoxide dismutasc (SOD), glutathione peroxidase (GSH-px), the content of malondialodchyde (MDA) and the release of lactate dehydrogenasc (LDH) The results showed that teh activity of SOD decreased and the activity of GSH-px, the content of MDA and the release of LDH increased following anoxia and reoxygenation. The actiwty or SOD appeared to be increased by either copper or zinc to varying degrees, but the effect of copper showed to be stronger than that of zinc. The release of LDH was reduced at the presence of lower amounts of copper or zinc, but the effect of zinc was better than that of copper. The increased release of LDH occured when higher amounts of copper or zinc were added to the medium. In addition, copper increased the activity of GSH-px while reducing the content of MDA significantly.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1990年第2期73-77,共5页
Chinese Journal of Pathophysiology
关键词
铜
锌
心肌缺氧
超氧物歧化酶
Copper
Zinc
Superoxidle dismutase
Myocardlium
Cell
