摘要
本实验利用离体工作心脏模型,在心脏停跳液中添加超氧歧化酶(SOD)和过氧化氢酶(CAT),观察对缺血后再灌注兔心功能和能量恢复的影响。动物随机分为两组,每组10只,对照组单纯应用St.ThomasⅡ号停跳液,实验组在停跳液中添加SOD20万U+CAT15万U/L。心肌缺血120min后,再灌注40min(主动脉逆行灌注10min,左心工作30min)期间,LVFSP、LVEDP、LV+dP/dtmaxLV-dP/dtmax的恢复百分比及ATP的含量均明显优于对照组(P<0.05或P<0.01)。提示外源性抗氧化酶SOD和CAT对缺血后再灌注心肌有一定的保护作用。
An experimental model of isolated rabbit working hearts was used to investigate whether supplementing cardioplegic solution with oxygen free radical scavengers exerts protective effect on ischemic and reperfusad heart.Animal hearts wero randomly divided into two groups : simple cardioplegic solution was used in the control group ; cardioplegic solution was supplemented with superoxide dismutase plus catalase in the experimental group.Following 120 min of ischemic arrest and 30 min of reperfusion, recoveries of LVPSP, LVEDP, LV + dp/dt max and LV-dp/dt max, expressed as percentage of preischemic control valu-S, and tissue ATP contjnt w-r significantly better (P<0.05 or 0.01) in the experimental group than that in the control group.The results of this study reveal that superoxide dismutase plus catalase enhances the efficacy of hypother-mic cadioplegia to protect the globally ischemic reperfused heart.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1989年第4期347-351,共5页
Academic Journal of Second Military Medical University
关键词
超氧物歧化酶
过氧化氢酶
心肌保护
oxygen free radical
isolated rabbit heart,myocarclial protection
