摘要
目的:研究水溶性猪牙皂多糖的抗氧化活性。方法:用水提醇分级沉降法和中空纤维素膜分离法制备不同相对分子质量的猪牙皂多糖。采用体外化学体系,以维生素C(Vit C)为阳性对照,测定猪牙皂多糖对羟自由基(.OH)、超氧自由基(O2-.)和过氧化氢(H2O2)的清除作用。结果:50%醇沉且相对分子质量在5 000～100 000的猪牙皂多糖对O2-.的清除活性高于Vit C。30%醇沉且相对分子质量大于10 000的猪牙皂多糖、50%醇沉且相对分子质量5 000～10 000的猪牙皂多糖、74%醇沉且相对分子质量大于5 000的猪牙皂多糖以及88%醇沉得到的猪牙皂多糖在0～1.00 g.L-1,随浓度的增加对.OH的清除作用逐渐增强,最大清除作用都可超过90%。不同醇沉不同浓度得到的猪牙皂多糖对H2O2有一定的清除活性,74%醇沉且相对分子质量大于5 000的猪牙皂多糖在0.07 g.L-1时,对H2O2的清除率为20.18%。结论:猪牙皂多糖具有良好的对.OH,O2-.和H2O2清除活性,猪牙皂多糖的抗氧化活性与醇沉浓度及其相对分子质量相关。
Objective：To study the antioxidation of polysaccharide extracted from Fructus Gleditsiae abnormalis.Method： The scavenging activities of the polysaccharide in vitro on hydroxyl radical（·OH）,superoxide radical（O-2·） and hydrogen peroxide（H2O2） were investigated by modified chemical systems.Result： The polysaccharide with 50% alcohol precipitation of the relative molecular mass of 5 000-100 000 performed the scavenging activities on superoxide radical higher than Vit C.Polysaccharide with 30% alcohol precipitation of the relative molecular mass more than 10 000,polysaccharide with 50% alcohol precipitation of the relative molecular mass 5 000-10 000,polysaccharide with 74% alcohol precipitation of the relative molecular mass more than 5 000 and polysaccharide with 88% alcohol precipitation presented hydroxyl radicals scavenging increasing with concentration increasing in 0.00-1.00 g·L-1 range.Also the largest scavenging rates were all more than 90%.The polysaccharides from Fructus gleditsiae abnormalis also showed certain scavenging activities on hydrogen peroxide,74% alcohol precipitation polysaccharide with relative molecular mass more than 5 000 scavenged 20.18% of hydrogen peroxide at 0.07 g·L-1.Conclusion： The polysaccharide extracted from Fructus Gleditsiae abnormalis can scavenge ·OH,O-2· and H2O2 effectively.The antioxidant potential of polysaccharides from Fructus Gleditsiae abnormalis is presumably related to their relative molecular mass.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第5期145-148,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30760304)
云南省科学研究基金项目(2006C0046M)
关键词
猪牙皂
多糖
抗氧化
活性
Fructus Gleditsiae abnormalis
polysaccharide
antioxidant
activity
