摘要
目的:研究多叶棘豆的清除自由基活性。方法:分别以常用抗氧化剂L-抗坏血酸和生育酚为对照,采用清除DPPH和ABTS自由基的方法对多叶棘豆乙醇提取物不同极性部位,以及AB-8大孔吸附树脂柱不同乙醇浓度洗脱部位进行清除自由基能力的评价。结果:多叶棘豆提取物AB-8大孔吸附树脂柱的50%乙醇洗脱部位对DPPH自由基和ABTS自由基均有较强的清除能力,其清除DPPH自由基能力强于同浓度的L-抗坏血酸。结论:AB-8大孔吸附树脂柱层析技术对多叶棘豆的清除自由基活性物质有分离富集作用,以50%乙醇洗脱部位活性最为突出。
Objective：To study DPPH and ABTS radical scavenging activities of Oxtropis myriophylla.Method： The anti-oxidant activity of O.myriophylla was measured by DPPH assay and ABTS assay.Furthermore,the AB-8 macroporous absorbent resincolumn chromatography with gradient elution（aqueous solution,50% and 90% ethanol solution）were used.Result：50% ethanol extract from O.myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions,which is a trifle stronger than ascorbic acid. In addition, the active ingredient was screened effectively by the column chromatography method. Conclusion：50% ethanol extract from O. myriophylla in the capability of scavenging DPPH and ABTS free radicals was much stronger than any other fractions, which is a trifle stronger than ascorbic acid. In addition, the active ingredient was screened effectively by the column chromatography method. Conclusion： The 50% ethanol extracts exhibited strong scavenging capacity of free radical.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期91-94,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
多叶棘豆
清除自由基活性
抗氧化
Oxtropis myriophylla
scavenging capacity of free radical
anti-oxidant
