摘要
为研究苏打碱胁迫对多年生黑麦草幼苗的生长和生理特性的影响,采用水培实验,用0.1mol/L的Na2CO3和NaHCO3溶液配置pH=8.2、pH=9.1、pH=9.9、pH=10.9四个梯度的处理液,以去离子水pH=6.5作为对照,探讨pH值对黑麦草蒸腾速率、株高、根长、含水量、叶绿素含量、丙二醛(MDA)含量以及抗氧化酶系统中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性的影响。结果表明,随着pH的升高,幼苗的株高、根长、含水量、相对蒸腾速率及叶绿素含量都显著下降,而根冠比呈先升后降的趋势。随着处理液的pH值从8.2递增至10.9,叶片中的MDA含量从对照的7.0nmol/g FW逐步上升到30.0nmol/g FW;而SOD活性先高后低,当pH=9.1时,最高含量为8.4U/mg蛋白;CAT的活性则从对照的118.5U/(min.g蛋白)逐步递减至47.1U/(min.g蛋白);而POD活性呈现递增的趋势,当pH达到最高时,POD活性是对照的4倍多。
The effects of saline sodic stress on growth and physiological responses of perennial ryegrass(Lolium perenne)were investigated in a greenhouse hydroponics experiment.Relative transpiration rate,shoot height,root length,water content,chlorophyll content,malondiadehyde(MDA)content were measured together with the activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD)of perennial ryegrass seedlings treated with one of five pH levels[6.5(The control),8.2,9.1,9.9,and 10.9 mixture of 0.1 mol/L Na2CO3,NaHCO3]and distilled water.As pH values increased,relative transpiration rate,shoot height,root length,water content,and chlorophyll content were significantly reduced compared with the control,while the ratio of root to shoot initially increased but then decreased.MDA contents increased(from 7.0 nmol/g FW to 30.0 nmol/g FW)with an increase of pH.At pH=9.1,SOD activity was the highest(8.4 U/mg protein).CAT activity was highest(118.5 U/min·g protein)at pH=6.5 and then declined at higher pH values.POD was lowest at pH=6.5 but increased as pH values increased.
作者
郭慧娟
胡涛
傅金民
GUO Hui-juan;HU Tao;FU Jin-min(Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,ChineseAcademy of Sciences,Wuhan 430074,China)
出处
《草业学报》
CSCD
北大核心
2012年第1期118-125,共8页
Acta Prataculturae Sinica
基金
公益性行业(农业)科研专项经费项目(200903001-2-4)资助
关键词
多年生黑麦草
苏打碱胁迫
生长状况
抗氧化酶
perennial ryegrass(Lolium perenne)
saline sodic stress
growth
antioxidant enzyme
