摘要
以谷氨酸棒杆菌(Corynebacterium glutamicum)CICC20887为生产菌株,采用单因素实验研究了发酵工艺条件对L-缬氨酸产量的影响.结果表明,该菌发酵生产L-缬氨酸的适宜初糖浓度、生物素添加量、VB1添加量、玉米浆添加量分别为90g/L、80μg/L、0.20mg/L、30g/L,发酵期间,24h前pH值应控制在6.5～6.7、后48h应控制在7.0～7.2,温度30～31℃,发酵周期应控制在66～72h.
Using Corynebacterium glutamicum CICC20887 as producing strain,the effect of fermentation process condition on the yield of L-valine were studied with single factor design;The results showed that the optimum concentration of initial glucose,D-biotin,VB1 and corn syrup were 90g/L,80μg/L,0.20 mg /L,30g/L respectively;The optimal fermentation conditions of pH,temperature and fermentation period were 6.5~6.7（for the initial 24 hours）,7.0~7.2（during 24 to 72 hours）,30~31℃,66~72 h respectively.This study could offe a basis for industrial production of L-valine.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第1期101-103,共3页
Journal of Central China Normal University：Natural Sciences
基金
湖北省教育科学"十一五"规划专项资助课题(2008B152)
咸宁职业技术学院课题(2009C008)
关键词
L-缬氨酸
发酵
谷氨酸棒杆菌
L-valine
fermentation
Corynebacterium glutamicum
