摘要
比较了采用不同方法处理链霉菌MIUG4．46的生物量时，细胞内葡萄糖异构酶的释放能力。当联合采用溶菌酶和高压力来破碎细胞时，提取物中葡萄糖异构酶的总酶活力为91．3％，酶的回收率为87％。用溶菌酶溶菌和高压破碎细胞后，在Mg^(2＋)和Co^(2＋)存在的情况下，对细胞悬浮液进行热处理（70℃下加热10min）时，有利于提高提取物中葡萄糖异构酶的纯度，酶的比活力达3．275U／mg蛋白质。
Some possibilities to release the glucose isomcrose were established for the selected strain streptomyces MIUG4. 46. when a combined treatment witb lysozyme and high pres sures has been applied to the cells. 91. 29% of the total enzymatic activity was found in the extract. and the yield for enzyme recovering was 87%. A thermal treatment (7O℃, 10min) applied to cells suspensions in the presence of Mg2+ and Co2+ions. after lysis with lysozyme and high pressures, determined a partial purification of extracts (the specific activity was 3. 275U /mg protein).
出处
《生物技术》
CAS
CSCD
1998年第6期18-21,5,共5页
Biotechnology
关键词
链霉菌
葡萄糖异构酶
提取条件
streptomyces, glucose isomerase, enzyme extraction,lysozyme
