摘要
对传统血清磷脂测定的抗坏血酸还原法进行了改良 ,大大减少了强酸的用量 ,其浓硫酸、过氯酸的用量只有改良前的 30 % ,消化时间缩短为改良前的 5 0 %～ 6 2 % ,血清用量减少为改良前的 40 %。改良后 ,血清磷脂测定结果的变异系数为 3.4%～ 3.7% ,回收率为 94%～ 1 0 9% ,平均回收率为 1 0 0 .8%。对 5 0份正常成人血清标本的磷脂测定结果表明 ,改良前后无显著性差异 (2 .81 3± 0 .333mm ol/ L 比 2 .882± 0 .31 6 mmol/ L,P>0 .0 5 ) ,其相关系数为 r=0 .991。上述结果表明 ,用改良抗坏血酸还原法测定血清磷脂含量 ,可大大减少强酸用量、缩短消化时间、减少血清用量 。
An antiscorbutate reduction method for serum phospholipids assay was modified by decreasing the consumed quantity of sulphuric acid and perchloric acid to 30% of that before modification, and the quantity of serum to 40% of that before modification. The digesting time was only 50%~62% of that before modification. The coefficients of variation (CV) were 3.4% and 3.7% for two batches of assays. Recovery of phosphotidylcholine added to serum ranged form 94% to 109%, averagely 100.8%. Serum phospholipids assay of 50 normal adults by the methods before and after modification showed statistically no significant difference (2.813±0.333mmol/L vs 2.828±0.316mmol/L, P>0.5) , but good correlation( r=0.991 ). The results indicate that the modified assay for serum phospholipids can decrease the use of serum and reagents, especially the harmful acids, and get the same result as that before modification.
出处
《华西医科大学学报》
CAS
CSCD
2000年第1期113-115,118,共4页
Journal of West China University of Medical Sciences
