摘要
以^(125)碘标记人不含apoE HDL_3为配体,研究纯化人apoAI、CⅠ、CⅡ、CⅢ、Cs及E对大鼠肝细胞膜HDL受体结合功能的影响。结果:非标记apoAI及apoCs均可有效竞争性抑制^(125)I-HDL_3与肝细胞膜HDL受体的结合;apoC亚类中,apoCⅢ抑制活性最强,抑制曲线与apoAI相似,非标记apoE则无明显抑制活性。表明apoC(尤其是apoCⅢ)对肝细胞膜HDL受体活性有重要调节作用,提示HDL受体可能是一种能识别和结合apoA及apoC的“apoA/C受体”。
Effects of purified humanapoliporoteins AⅠ, CⅠ, CⅡ, CⅢ_(-1),, Csand E on the binding of ^(125)Ⅰ.-labeled apoE-deficient HDL, to isolated rat liverplasma membranes were investigated.Unlabeled apo AⅠ, CⅠ, CⅡ, CⅢ_(-1), andapo Cs, but not apo E, could effectivelyinhibit ^(125)Ⅰ-labeled HDL_3 binding to livermembranes. apoCⅢ_(-1) was the strongestamong the apoC subclasses and the inhi-bition curve of apoCⅢ _(-1)was similar tothat of apo AⅠ. This result indicates thatapo C, especially apo CⅢ, may be ano-ther specific ligand of HDL receptor, andit plays an important role in regulationof HDL receptor activity in liver.
出处
《华西医科大学学报》
CSCD
1991年第2期120-123,共4页
Journal of West China University of Medical Sciences
基金
国家攻关资助
关键词
载脂蛋白
肝细胞膜
脂蛋白受体
Apolipoproteins
Liver plasma membranes
High-density-lipoprotein receptor
