摘要
为探讨高三尖杉酯碱对人眼球后成纤维细胞 (HROF) )增殖的影响 ,采用体外培养人眼球后成纤维细胞 ,用 MTT比色法测定人眼球后成纤维细胞的增殖程度 ,并观察高三尖杉酯碱对 HROFb生长和增殖的影响。结果显示 :MTT比色法能精确反映 HROFb的增殖程度 ,所测光吸收值与细胞数目成正相关 (r=0 .996 ,P<0 .0 0 1 )。高三尖杉酯碱可抑制 HROFb的增殖 ,细胞抑制率与高三尖杉酯碱浓度呈显著正相关 (r=0 .6 932 ,P<0 .0 0 1 )
This is a study amied at the effects of homoharringtonine on the proliferation of human retro ocular fibroblasts. The human retroocular fibroblasts (HROFb) were cultured and MTT colorimetry assay was used to determine the proliferation of HROFb. The influence of homoharringtonine on the proliferation of HROFb was observed in vitro. The results showed that MTT colorimetry assay could precisely reflect the proliferation of HROFb. The absorbance was directly proportional to the number of the cells ( r=0.996, P<0.001) . The proliferation of HROFb was inhibited by the homoharringtonine and the cell proliferation percentage was correlated positively to the homoharringtonine level ( r=0.6932, P<0.001) .
出处
《华西医科大学学报》
CSCD
2000年第1期95-97,共3页
Journal of West China University of Medical Sciences
基金
四川省卫生厅科研基金!资助 (课题编号 F0 13 8)
关键词
GRAVES眼病
成纤维细胞
高三尖杉酯碱
Grave's ophthalmopathy\ \ Fibroblast MTT\ \ Colorimetry assay\ \ Homoharring tonine
