摘要
目的 探讨地塞米松治疗Graves眼病 (GO)的作用机制。方法 应用细胞培养技术 ,测定加入细胞因子及地塞米松后细胞间粘附分子 1(ICAM 1)表达和DNA合成情况。结果 肿瘤坏死因子 (TNF) α(10 3 U/ml)、干扰素 (INF) γ(10 0U/ml)和白细胞介素 (IL) 1β(10 0U/ml)均能强烈刺激眼球后成纤维细胞表面ICAM 1表达 ,而地塞米松能明显地抑制这种刺激作用 ,但所需浓度及发挥抑制作用的时间各不相同 ;同样 ,地塞米松也能抑制细胞因子刺激的DNA合成。结论 地塞米松治疗GO的作用途径部分是通过抑制细胞因子的作用 ,使ICAM 1表达及DNA合成减少。
Objective To investigate the mechanism by which dexamethasone treats Graves' ophthalmopathy (GO). Methods ICAM 1 expression and DNA synthesis in cultured retroocular fibroblasts incubated with cytokines and dexamethasone were measured. Results Some cytokines such as TNF α (10 3 U/ml)、IFN γ(100 U/ml) and IL 1β (100 U/ml) could significantly stimulate the ICAM 1 expression in retroocular fibroblasts. Dexamethasone markedly inhibited these stimulations, but the effective concentrations and time to these cytohious varied. Dexamethasone also could inhibit the DNA synthesis stimulated by these cytokines. Conclusion Dexamethasome treats GO partially by inhibiting the effect of cytokines and decreasing the expression of ICAM 1.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2000年第4期224-227,共4页
Chinese Journal of Endocrinology and Metabolism
