摘要
目的 :了解异丙酚对大鼠脑一氧化氮 (NO) /环鸟苷酸 (cGMP)信号转导系统的影响。方法 :32只SD大鼠 ,随机分为对照组和异丙酚组 ,分别腹腔注射 (ip)生理盐水 10ml/kg或异丙酚 10 0mg/kg。用分光光度法测定脑组织一氧化氮合酶 (NOS)活性和NO产量 ,放射免疫法测定脑组织cGMP含量。结果 :与对照组比较 ,大鼠ip异丙酚 10 0mg/kg不但明显抑制小脑、海马和大脑皮层的NOS活性 ,而且显著减少上述脑区NO产量和cGMP含量 (P <0 0 5或P <0 0 1)。结论
Objective: To investigate the effects of propofol on NO/cGMP signal pathway in rat brain.Methods:32 SD rats were divided randomly into control group and propofol group and either normal saline 10ml/kg or propofol 100mg/kg was administered introperitoneally(ip).Nitric oxide synthase(NOS) activity and NO production were assessed by spectrophotometric analysis.cGMP content was measured by radioimmunoassay.Results:Propofol 100mg/kg significantly inhibited NOS activity and reduced NO production and cGMP content of the cerebellum,hippocampus and cerebral cortex as compared with those of control group (P<005 or P<001).Conclusion:NO/cGMP signal pathway may play an important role in the molecular mechanism of propofol anesthesia.
出处
《临床麻醉学杂志》
CAS
CSCD
2000年第2期93-95,共3页
Journal of Clinical Anesthesiology
基金
国家自然科学基金资助项目!(编号:39670700)
关键词
异丙酚
一氧化氮
环鸟苷酸
信号转导
大鼠
Propofol Nitric oxide Cyclic guanosine monophosphate Signal pathway
