摘要
Objective To investigate the effect of propofol on hippocampal Bcl-2 protein expression after forebrain ischemia/ reperfusion (I/R) in rats. Methods forty-two male Wistar rats weighting 250 g^300 g were randomly divided into three groups of fourteen animals: control group (C)in which sham operation was performed; I/R group (I) in which bilateral common carotid arteries were clamped for 10 mins combined with hypotension(MAP:35 mm Hg～45 mm Hg)induced by exsanguinations, then 1 ml/kg saline was injected into the lateral cerebral ventricle using microsyringe; propofol group (P)in which 1 mg/kg propofol was injected into the lateral cerebral ventricle using microsyringe after I/R. The end of 24 h reperfusion the animals were decapitated, then coronal tissue sections and hippocampal tissue were obtained for detection of Bcl-2 protein expression by immuno-histochemistry technique(n = 8)and bcl-2 mRNA expression by RT-PCR technique(n = 6). Results The expression of Bcl-2 protein in group P increased significantly compared with that in group C and I (P < 0.05). The bcl-2 mRNA expression in group P was enhanced compared with that in group C and I (P < 0.05). Conclusion Propofol can enhance the Bcl-2 protein expression and increase the bcl-2 mRNA expression after forebrain ischemia-reperfusion in rats.
Objective To investigate the effect of propofol on hippocampal Bcl-2 protein expression after forebrain ischemia / reperfusion （I/R） in rats. Methods forty-two male Wistar rats weighting 250 g - 300 g were randomly divided into three groups of fourteen animals： control group （C）in which sham operation was performed; I/R group （I） in which bilateral common carotid arteries were clamped for 10 mins combined with hypotension（ MAP：35 mm Hg-45 mm Hg）induced by exsanguinations, then ! ml/kg saline was injected into the lateral cerebral ventricle using microsyringe; propofol group （P）in which 1 mg/kg propofol was injected into the lateral cerebral ventricle using microsyringe after I/R. The end of 24 h reperfusion the animals were decapitated, then coronal tissue sections and hippocampal tissue were obtained for detection of Bcl-2 protein expression by immuno-histochemistry technique（ n = 8） and bcl-2 mRNA expression by RT-PCR technique（ n = 6）. Results The expression of Bcl-2 protein in group P increased significantly compared with that in group C and I （ P 〈 0.05）. The bcl-2 mRNA expression in group P was enhanced compared with that in group C and I （P 〈 0.05）. Conclusion Propofol can enhance the Bcl-2 protein expression and increase the bcl-2 mRNA expression after forebrain ischemia-reperfusion in rats.
出处
《国际麻醉学与复苏杂志》
CAS
2006年第4期193-196,共4页
International Journal of Anesthesiology and Resuscitation
基金
国家自然科学基金(项目编号:30240023)
中国博士后科学基金(项目编号:2003034343)
教育部归国人员科研启动基金
华西-宜昌人福药业麻醉学论坛青年医师奖学金(2003年度)
