摘要
目的：探讨躯体性及心理性应激状态下大鼠胃粘膜ＮＯＳ活性变化及其与胃粘膜损伤的关系。方法：将９６ 只雄性ＳＤ大鼠随机分为３组：对照组，规则光（Ｒ）组及不规则光（Ｉ）组，采用高压恒流脉冲刺激器制备躯体性和心理性应激模型。分光光度法检测胃粘膜组织的ＮＯＳ活性和Ｎｉｌｓ Ｌａｍ ｂｅｃｈｔ法测定胃粘膜损伤指数。结果：正常情况下ＮＯＳ活性平稳，应激后大鼠胃粘膜ＮＯＳ活性均明显升高，Ｉ组升高程度更明显；与对照组比较，Ｒ，Ｉ组胃粘膜损伤指数均较高，且Ｉ组较Ｒ组变化显著（Ｐ＜ ０．０５）。结论：心理性应激程度愈高，胃粘膜损伤愈严重。
Objective: To explore the relation between the activity changes of nitric oxide synthase and acute gastric mucosal lesion in gastric mucosa under physical and psychological stress. Methods: Animal model was constructed with impulsive stimulator of high voltage and stable currency. Ninety six male SD rats were randomly divided into 3 groups: control, regular and irregular group. NOS activity was measured by spectrophotometry and injure of gastric mucosa was measured by method of Nils Lambecht. Results: NOS activity was stable in normal rats but increased significantly in group of psychological stress( P <0.05); gastric mucosa injury were observed in regular group, irregular group and the later was server( P < 0.05). Conclusion: The degree of increase of the NOS activity and gastric mucosa injury are positively correlated to the severity of psychological stress.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第1期10-12,共3页
Academic Journal of Second Military Medical University
基金
军队医药卫生杰出中青年人才科研基金!资助项目(1996 卫科训字95 号)
