摘要
本研究采用肿瘤细胞与小鼠腹腔巨噬细胞混合培养的方法,以Griess′s法测定一氧化氮(NO)产量,MTT法测定肿瘤细胞活力。结果发现多抗甲素(PolyactinA,PAA)对小鼠巨噬细胞NO的释放有促进作用,同时可在体外通过NO直接杀伤肿瘤细胞。
Tumor cells cultured with mice macrophages for a few hours, then detected the production of nitro oxide with Griess′s method and evaluated the tumor cell activity with MTT method. Results showed that polyactin A significantly induced nitro oxide production from mice macrophages and the nitro oxide can inhibit the tumor cell′s activity remarkably.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2000年第1期62-63,共2页
Chinese Journal of Antibiotics
基金
华西医科大学青年基金
