摘要
目的:研究多抗甲素(PA)与GM-CSF,TNF-α,IL-4(GTI)联用对脐血单个核细胞(CBMC)杀瘤效应的影响。方法:①分别用PA,GTI及GTI+PA(GTIP)体外诱导培养CBMC,免疫组织化学法检测细胞表型变化,以CD1a和CD83作为树突状细胞(DC)特异性标记;②GTI体外培养CBMC24h后,分别加入凋亡坏死的Hela和HepG2细胞共孵育2d,再加入PA,3d后以处理后的CBMC作为效应细胞(另设未加凋亡坏死肿瘤细胞组、未加PA组为对照),以相应的肿瘤细胞为靶细胞,用MTT法测定不同效靶比(10∶1,20∶1)下效应细胞对Hela和HepG2细胞的杀伤率。结果:①培养第7天,PA组CD1a+和CD83+细胞比率分别达(19.63±3.61)%和(9.28±4.31)%,显著高于对照组,但明显低于GTI组;②GTI与PA联用后,CBMC对Hela和HepG2细胞的杀伤率显著提高,明显高于对照组,GTI组及PA组;而与凋亡坏死瘤细胞共孵育后,杀伤率进一步提高。结论:PA能促进脐血DC体外扩增、成熟;PA不仅可协同GTI增强CBMC对肿瘤的杀伤率,还可增强GTI作用下负载肿瘤细胞抗原的CBMC对肿瘤杀伤率。
Objective To investigate the tumor-suppression effect of PA combined with GM-CSF, TNF-α and IL-4 on cord blood mononuclear cells （CBMC）. Methods The mononuclear cells were isolated from human umbilical cord blood and cultured with polyacttin A （PA） , GM-CSF ＋ TNF-α ＋ IL-4 （GTI） , and GTI ＋ PA （GTIP） respectively. Six days later, surface antigen expression of the cultured cells, including CD1 a and CD83, which were the specialized markers of dendritic cell （DC） , were analyzed by immunohistochemistry technique. The CBMC were cultured with GTI for 24 h to enhance DC, then were added apoptotic/necrotic Hela/HepG2 tumor cells, and finally PA was co-cuhured. The antitumor cytotoxicity of CBMC was measured by MTF assay. Results After the culture, CDla and CD83 positive cell rates of the PA group increased significantly, reaching （ 19. 63 ＋ 3. 61 ）% , （9. 28 ＋ 4.31 ） % respectively, much higher than that of the control, but lower than that of the GTI group. The killing rate to the tumor cells of CBMC cultured with GTIP increased remarkably, much higher than the control, GTI and PA groups. After tumor antigens were added to the CBMC of GTIP group （GTIP ＋ Tc）, the killing rate increased. Conclusion PA not only promotes the proliferation and maturation of cord blood derived DC, but also improves the tumor-suppression effect of CBMC cultured with GTI.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期553-557,共5页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(30400515)
