摘要
研究了欧李仁综合利用的技术路线,通过非热方法控制欧李仁油、苦杏仁苷和欧李仁蛋白3种成分分离提取过程中苦杏仁苷酶的催化活性来实现三者的全利用。结果表明:在欧李仁破碎和欧李仁油提取时,通过控制水分活度抑制其催化活性,适宜的水分活度为0.67;继而在苦杏仁苷和欧李仁浓缩蛋白分离提取时,通过控制乙醇体积分数和温度抑制其催化活性并防止欧李仁蛋白过度变性,乙醇体积分数和温度以75%、45℃为最佳;采用以上条件对欧李仁油、苦杏仁苷和欧李仁浓缩蛋白进行分离提取,提取率分别达到96.98%、73.04%和90.29%,其中浓缩蛋白的氮溶解指数为69.86%;供制备苦杏仁苷的欧李仁原料储藏时水分活度应控制在0.67,以防止苦杏仁苷在内源酶催化下发生水解。
The multipurpose use of Cerasus humilis kernel was studied to obtain the oil, amygdalin and protein. In order to obtain the three products,it was essential to inhibit the catalytic activity of amygdalase using non - thermal processing technology. In the process of crashing the kernel and extracting the oil, the catalytic activity was inhibited by controlling the water activity at 0. 67. In the process of extracting theamygdalin and protein, the catalytic activity was inhibited and the protein denaturation was prevented by controlling the volume fraction and temperature of ethanol at 75 % and 45℃, respectively. Under the op-timal conditions the extraction rate of the oil, amygdalin and protein concentrate was 96.98% ,73.04% and 90.29%, respectively, and the NSI of the protein was 69.86%. The amygdalin of the kernel could behydrolyzed by the endogenous amygdalase, so the Cerasus humilis kernel, used as raw materials to produce amygdalin, should be stored at 0. 67 of the water activity to prevent the hydrolysis.
出处
《中国油脂》
CAS
CSCD
北大核心
2012年第2期65-69,共5页
China Oils and Fats
基金
北京市农林科学院科技创新能力建设专项(KJCX201101011)
关键词
欧李
欧李仁
苦杏仁苷
欧李仁油
欧李仁蛋白
Cerasus humilis
Cerasus humilis kernel
amygdalin
Cerasus humilis kernel oil
Cerasus humilis kernel protein
