摘要
将玉米赤霉烯酮转变成玉米赤霉烯酮-6’-羧甲氧肟,通过混合酸酐法将其与牛血清白蛋白结合并用以免疫兔获得抗体。抗体效价可达1:4×10~4,亲合常数为4.25×10^(10)L/mol,灵敏度提高为3.5pg。样品平均回收率达92％。批内与批间变异系数分别为6.1％和8.6％。
Zearalenone RIA was established and used to detect zearalenone in plants. Zeazalenone wa first converted into zearalenone-6'-carboxymethyloxime, and then identified by TLC, NRM and MS/MS, and conjugated with bovine serum albumin (BSA). The molecular ratio of zearalenone to BSA was about 23:1 as determined by UV scanning analysis. After five months of immunization of rabbit, the antibody titers reached 1:4×10~4. The cross reaction of this antiserum was very low (less than 0.05%) for phytohormones, but much higher for analogues of zearalenone, such as zearalanol. The Ab avidity wa 4.25×10~10 L/mol, and the detection limit was 3.5 pg. CV% of RIA in each sample batch and between sample batches was 6.1% and 8.6% respectively.
Zearalenone in different plant samples was assayed using this method and the results indicated that zearalenone, as an endogenous active substance, occurred widely in higher plants.
基金
国家自然科学基金
关键词
玉米赤霉烯酮
放射免疫测定
春化
zearalenone, radioimmuncassay(RIA), vernalization
