摘要
目的:定量检测乳腺癌患者血清中的游离DNA,并评估其应用前景。方法:采用荧光定量PCR方法检测100例健康女性志愿者、100例乳腺良性疾病患者和200例乳腺癌患者血清游离DNA中GAPDH基因拷贝数,以此作为评估DNA含量的指标。结果:以≥1×103基因拷贝数作为阳性标准,93%健康女性和95%乳腺良性疾病患者DNA检测为阴性,而84.5%乳腺癌患者DNA检测为阳性,其中Ⅰ～Ⅱ期乳腺癌患者阳性率为84%。DNA含量在3组之间的差异有统计学意义(P<0.005)。结论:早期乳腺癌患者血清中存在一定数量肿瘤源性的游离DNA,此类遗传物质可以作为一类特异性的生物学标志物。
Objective： To quantitatively detect the serum circulating DNA in patients with breast cancer and evaluate its potential applications. Methods： The copy number of GAPDH gene in serum circulating DNA was measured by real-time quantitative PCR from 100 healthy female volunteers, 100 patients with benign breast lesions, and 200 patients with breast cancer. The copy number of GAPDH gene was used as an indicator for circulating DNA content. Results： With a cut-off value of 〉11 ~103 copies, the results of serum circulating DNA detection in 93% of healthy females and 95% of patients with benign breast lesions were negative, and 84.5% of patients with breast cancer were positive. The positive rate of serum circulating DNA in patients with 1-11 stage breast cancer was 84%. The DNA content was significantly different among healthy females, patients with benign breast lesions and the patients with breast cancer （P〈0.005）. Conclusion： There is a certain amount of tumor-derived circulating DNA in patients with early stage breast cancer. These genetic materials may serve as a type of specific biomarkers.
出处
《肿瘤》
CAS
CSCD
北大核心
2011年第12期1099-1102,共4页
Tumor
基金
上海市科学技术委员会科研计划项目(编号:08411966800)
关键词
乳腺肿瘤
血清
生物学标记
游离DNA
Breast neoplasms
Serum
Biological markers
Circulating DNA
