摘要
目的探讨血清游离p16INK4A基因启动子DNA甲基化用于肺癌早期诊断的临床应用价值。方法选择32例健康查体者,32例肺部良性疾病患者和32例Ⅰ期(T1N0M0或T2N0M0)非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清游离DNA做为研究对象,应用甲基化特异PCR(methylation specific PCR,MSP)对上述标本进行p16INK4A基因启动子DNA甲基化检测,判断各组标本启动子的甲基化水平。评价血清游离DNA启动子异常甲基化对于NSCLC早期诊断的价值。结果健康对照组血清游离p16INK4A基因启动子DNA甲基化均呈阴性;肺部良性病变组甲基化阳性率为25.0%(8/32);NSCLC患者组甲基化阳性率为62.5%(20/32)。3组标本血清游离p16INK4A基因启动子DNA甲基化阳性率差别有统计学意义(χ2=16.54,P=0.033)。以血清游离p16INK4A基因启动子DNA甲基化为肺癌诊断标准,其诊断NSCLC的敏感度和特异性分别为59.4%,87.5%。诊断的ROC(receiver operating characteristic)曲线下面积(AUC)为0.86,95%CI(0.74～0.95)。结论与健康体检者和肺部良性病变患者相比,NSCLC患者血清游离p16INK4A基因启动子DNA甲基化水平显著增高,检测血清p16INK4A基因启动子甲基化水平可能为肺癌早期诊断提供一种无创的方法。
Objective To evaluate the diagnostic effect of serum DNA methylation in p16INK4A gene for stage I non - small cell lung can cer（NSCLC）. Methods We seperately selected 32 healthy people, 32 cases with no malignant lung diseases and 32 cases with NSCLC. The methylation leveal of the specimens in p16INK4A gene was examind by using methylation - specific PCR. Results In patients with NSCLC, methylation was detected in 20 patients（62.5% ） ,which was higher compared with that in patients with healthy people（0% ） and non - malignat lung diseases （25.0%） （X2 = 16. 54, P =0. 033）. The sensitivity and specificity for NSCLC in of p16INK4A gene promoter methylation were 59.4% , 87.5%, respectively. The under- curve -area （AUC） of p16INK4A gene promoter methylation for diagnosis of NSCLC was 0.86,95% CI（0.74,0.95 ）. ConcLusion The methylation level of p16INK4A gene prometer was much higher in NSCLC patients. So, the identification of promoter methylation of p16tNK4A gene in serum DNA could be useful for detection of early stage NSCLC.
出处
《医学研究杂志》
2013年第4期163-166,共4页
Journal of Medical Research
