摘要
目的探讨乳腺单纯性导管增生(UDH)、乳腺不典型导管增生(ADH)和浸润性导管癌(IDC)样本中位于3p、17p和17q的5个微卫星DNA(MS)位点杂合性缺失(LOH)的频率和模式。方法采用显微分离技术分离病变细胞,聚合酶链反应扩增D3S1029、D3S1300、TP53、D17S579、D17S855目的DNA片段。结果 (1)UDH组D3S1029、D17S855位点LOH频率分别为9.1%和8.3%;ADH组D3S1029、D3S1300、D17S579、D17S855位点LOH频率分别为25.0%、18.2%、10.0%1、6.7%,TP53位点未见LOH;IDC组五个MS位点的LOH频率均大于25%。(2)IDC组TP53和D17S579位点LOH的频率显著高于ADH组(P<0.05);IDC组5个MS位点LOH的频率显著高于UDH组(P<0.05);IDC组发生多个位点LOH的频率显著高于ADH和UDH组(P<0.05)。结论 (1)5个MS位点的LOH频繁发生于散发性乳腺癌;(2)部分UDH、ADH发生D3S1029、D3S1300、D17S579、D17S855位点的LOH,是否作为乳腺癌前损伤恶变风险的分子标志,需进一步研究。
Objective To study loss of heterozygosity（LOH） frequency and pattern of the 5 MSs on 3p、17p、17q in unilateral ductal hyperplasia（UDH）,atypical ductal hyperplasia（ADH） and invasive ductal carcinoma（IDC） groups.Methods （1） Microdissect normal control cells and sick cells from HE stained pathological sections;（2） Amplify objective DNA fragments of D3S1029、D3S1300、TP53、D17S579、D17S855 by polymorphism chain reaction（PCR）.Results（1） In UDH group,the frequencies of LOH at D3S1029 and D17S855 were 9.1% and 8.3% separately;In ADH group,the frequencies of LOH at D3S1029,D3S1300,D17S579 were 25.0%,18.2%,10.0%,16.7% separately,and there was no LOH at TP53 in this group;In IDC group,the frequencies of LOH at 5 MS were all over 25%.（2）There were significant differences between ADH and UDH,ADH and IDC groups（P0.05）,and significant differences between UDH and IDC,ADH and IDC groups（P0.05）.There were significant difference between UDH and ADH,UDH and IDC groups（P0.05）.Conclusion （1） LOH of the 5 MS loci is frequent in sporadic breast cancer.（2） Whether the LOHpresented at D3S1029,D3S1300,D17S579,D17S855 loci could be regarded as risk factors of putative precursors of breast cancer needs to be studied further.
出处
《中国实验诊断学》
北大核心
2011年第4期592-595,共4页
Chinese Journal of Laboratory Diagnosis
