摘要
目的探讨γ－谷氨酰转移酶（ＧＧＴ）异常表达在监测肝细胞癌变中的价值。方法以２－乙酰氨基芴（２－ＦＡＡ）喂饲ＳＤ大鼠诱发肝癌，观察鼠肝细胞在癌变期间的病理组织学、酶组织化学、肝总ＲＮＡ水平、不同分子形式ＧＧＴ及同工酶的动态改变。结果诱庙后随鼠肝组织学改变，肝ＧＧＴ过度表达，可溶性及膜结合性ＧＧＴ比活性（Ｕ／ｇ）均明显异常（ｑ＝４．９０， Ｐ＜０．０１） ；肝总 ＲＮＡ浓度与肝ＧＧＴ比活性或血清ＧＧＴ活性均呈显著正相关（ｒ＝０．９０， ｒ＝０．７９， Ｐ＜０．０１）；同工酶分析显示实验鼠出现对照鼠和胚胎鼠的混合谱型，于诱癌初期的鼠肝和血中可检出癌胚性ＧＧＴ。结论ＧＧＴ为反映肝细胞癌变敏感标志酶，癌胚性ＧＧＴ形式的分析有助于鼠肝癌的早期诊断。
Objective To explore the value of gama-glutamyl transferase (GGT) abnormal expression in monitoring carcinogensis of hepatocytes. Methods The kinetic alterations of rat (SD) liver pathology, enzymatic histochemistry, total RNA levels, different molecular form GGT and its isoenzymes were inveshgated on experimental hepatomas inducing with 0. 05% 2-fluoenylacetandde (2-FAA). Results The GGTs of livers were overexpressed ddring canceration, liver GGT specific achvihes (Ulg) including soluble and membrane-combined GGT achvihes of each group in experiment rats were significantly higher (q = 4.90, p < 0.01) than those of control groan. The total RNA concentalon was highly positively correlated with liver GGT specific achvities or serum GGT activities (r = 0.90, r = 0.79, P < 0.01). The mixed isoenzyme patterns of control rats' and embryo rats' GGT were found in sera and livers of experimental rats, the carcino-embryonic GGT bands can be detected at early stages of rat hepatocyte canceration. Conclusions The present data suggest that GGT is a sensitive enzyme for carcinogensis of hepatocytes, the analysis of carcino-embryonic GGT is a useful marker for early diagnosis of rat hepatoma.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第1期30-32,共3页
Chinese Journal of Hepatology
基金
江苏省科委!BS98029
江苏省卫生厅重点项目!H9807
关键词
肝细胞癌
诊断
Γ谷氨酰转移酶
同工酶
Carcinoma, hepatocellular
Diagnosis
Gama-glutamyl transferase
Isoenzymes
