摘要
以提取和纯化的肝癌特异性ＧＧＴ（ＧＧＴ－Ⅱ）免疫新西兰兔，制备得抗血清。建立肝癌特异性ＧＧＴ火箭电泳测定法，对原发性肝癌（ＰＨＣ）及其他非癌性肝病等患者检测。此法对ＰＨＣ诊断的敏感性和特异性分别为８６．９的和９２．１％，在早期和ＡＦＰ阴性的ＰＨＣ中，亦具有较高的阳性率。结果表明，本文所建立的测定法对于诊断ＰＨＣ，包括早期和ＡＦＰ阴性的ＰＨＣ具有定性诊断价值和应用前景。
New Zealand rabbit was immunized by purified
hepatoma-specific GGT(GGT-Ⅱ)as antigen,and the rabbit antiserum was prepared.Rocket
immunoelectrophoresis assay using the antiserum against hepatoma-specific GGT(GGT-Ⅱ)was
established,and the patients with PHC and other non-neoplastic liver diseases were
studied.The diagnostic sensitivity and specificity for PHC of this immunoassay were 86.9%and
92.1% respectively, and there were high positive rates in early PHC and AFP negative PHC. It
was shown this immnunoassay had qualitative diagnostic value for PHC(included early and
AFP negative PHC)and application prospects.
出处
《中华消化杂志》
CAS
CSCD
北大核心
1995年第5期269-271,共3页
Chinese Journal of Digestion
基金
江苏省应用基础研究计划资助
