摘要
目的 探讨肝窦内皮细胞细胞因子表达在脓毒症小鼠肝损伤中的作用。方法 采用盲肠结扎穿孔(CLP)制造小鼠脓毒症模型,假手术组(Sham)接受同样的手术操作但不行CLP。分别在致伤后3,12h分离肝窦内皮细胞,用RT-PCR的方法定量检测肝窦内皮细胞中肿瘤坏死因子α(TNFα),白细胞介素1β(IL1β)及白细胞介素6(IL6)的基因表达。结果 肝窦内皮细胞TNFα,IL1β基因表达在CLP后3h即明显增高,至12h时虽有所降低但仍明显高于Sham组;IL6基因的表达在CLP后3h明显增高,至CLP后12h仍维持在高水平。结论 肝窦内皮细胞是脓毒症时肝组织中细胞因子的重要来源之一。
Objective To investigate the role of cytokines genes expression of liver sinusoidal endothelial cells in liver damage by sepsis.Methods Septic mice models were established with cecal ligation and perforation (CLP), while sham operation group received the same procedure exclusive CLP. The genes expression of TNFα, IL 1β and IL 6 in liver sinusoidal endothelial cells were assessed by RT PCR. Results A significant increase of TNFα, IL 1β genes expression was observed at 3h, and a slight decline at 12h after operation, but still significantly higher than that in the sham group; while IL 6 gene expression showed signficantly higher at 3h and remained at the high level at 12h. Conclusions Liver sinusoidal endothelial cell is an important source of cytokine production in mice with sepsis.
出处
《中国普通外科杂志》
CAS
CSCD
2000年第1期32-34,共3页
China Journal of General Surgery
基金
国家自然科学基金资助(39870796)
