摘要
目的 探讨脓毒症小鼠肝损伤时肝窦内皮细胞粘附分子基因表达变化。方法 用盲肠结扎穿孔 (CLP)法制造小鼠脓毒症模型 ,假手术组 (sham)接受同样的手术操作但不行CLP。分别在致伤后 3、12h分离肝窦内皮细胞 ,用RT PCR的方法定量检测E selectin、ICAM 1和VCAM 1的基因表达 ,同时还测定了肝组织中MPO活性及微血管通透性的改变。结果 CLP后 3h肝窦内皮细胞E se lectin基因的表达明显增高 (0 85± 0 0 6vs.0 2 2± 0 0 4,P <0 0 1) ;ICAM 1基因的表达在CLP后 3h即明显增高 12h时达高峰 (1 0 2± 0 10vs.0 30± 0 0 4,P <0 0 1) ;VCAM 1基因的表达在CLP后 3h即明显增高 (1 0 4± 0 14vs.0 37± 0 0 4,P <0 0 1) ,至CLP后持续到 12h后肝组织中MPO活性在CLP后 3h明显增高 (0 5 89± 0 116vs.0 16 0± 0 0 2 5 ,P <0 0 1) ,持续到 12h。CLP后 3h肝微血管通透性即开始升高 ,至 12h时与sham组比较差异有显著意义 (0 0 49± 0 0 0 7vs .0 0 34± 0 0 0 4,P <0 0 1)。结论 脓毒症小鼠肝窦内皮细胞粘附分子表达增高可能是导致白细胞在损伤组织局部聚集进而造成器官功能损伤的重要因素。
Objective[WT5”BZ] To investigate the adhesion molecules gene expression in murine sepsis induced liver injury.[WT5”HZ] Methods[WT5”BZ] Sepsis was induced in mice by cecal ligation and puncture(CLP). Sham operation group was used as control. The gene expressions of adhesion molecules (E selectin, ICAM 1 and VCAM 1) in liver sinusoidal endothelial cells were assessed by RT PCR. Changes of MPO and microcirculatory permeability in the liver were measured.[WT5”HZ] Results[WT5”BZ] A significant increase in E selectin gene expression was observed at 3 hours after CLP 0 85±0 06 vs. 0 22±0 04 ( P <0 01). And the gene expressions of ICAM 1 and VCAM 1 significantly increased between 3 and 12 hours after CLP 1 02±0 10 vs. 0 30±0 04( P <0 01),and 1 04±0 14 vs. 0 37±0 04( P <0 01). A significant increase in hepatic tissue MPO was also found between 3 and 12 hours after CLP 0 589±0 116 vs. 0 160±0 025 ( P <0 01).The microcirculatory permeability in the liver began to increase at 3 hours after CLP, and this increase became significant at 12 hours after CLP 0 049±0 007 vs. 0 034±0 004 ( P <0 01).[WT5”HZ] Conclusion[WT5”BZ] The up regulation of the gene expressions of adhesion molecules in liver sinusoidal endothelial cells may lead to the recruitment of leukocytes at the site of inflammation causing liver injury during sepsis.[WT5”HZ]
出处
《中华普通外科杂志》
CSCD
2000年第5期297-299,共3页
Chinese Journal of General Surgery
基金
国家自然科学基金!(39870 796)
关键词
脓毒症
细胞间粘附分子
肝损伤
小鼠
WT5”BZ] Sepsis
Endothelial growth factors
Intercellular adhesion molecule 1
