摘要
目的：研究中药复方ＡＣ９６０对人肝癌细胞基因、抑癌基因表达的影响，探讨其抗癌作用机制。方法：ＡＣ９６０灌胃给药后，采集大鼠血清，处理人肝癌细胞ＳＭＭＣ７７２１，３Ｈ－ＴｄＲ掺入法观察细胞增殖，流式细胞仪分析细胞周期和ｐ２１ｒａｓ、ｂａｌ－２、Ｐ５３基因蛋白表达。结果：ＡＣ９６０具有显著抑制 ＳＭＭＣ－７７２１细胞增殖和促进细胞凋亡作用，细胞周期分析，ＤＮＡ直方图上见典型凋亡峰，２４ｈ时为２１．２５％，４８ｈ时为２７．７７％，早期（２４ｈ）Ｓ期细胞比例增加，Ｇ２／Ｍ期减少，后期（４８ｈ），Ｇｏ／Ｇ１期细胞增多，Ｓ期减少。Ｐ５３、ｂａｌ－２、ｐ２１ｒａｓ在 ＳＭＭＣ－７７２１细胞中都有不同程度表达，ＡＣ９６０处理后，Ｐ５３表达增强，ｂｃｌ－２、ｐ２１ｒａｓ表达降低。结论：抑制癌基因、增强抑癌基因表达，阻断细胞增殖周期，抑制人肝癌细胞增殖，促进癌细胞凋亡可能是ＡＣ９６０抗肝癌主要作用机制。
Objective:To study the effects of AC960 on the expression of oncogene and tumor suppressor gene and investigate its anti - cancerous mechanism. Methods:Drugcontending serum was derived from rats orally pre-administered with AC960 through gastrogavage,and was added to subcultured humand hepatoma cells(SMMC-7721 )while the normal rat serum were as the control. Cell proliferation was measured with3H- TdR incorporation Cell apoptosis and expressions of P53,bcl- 2 and p21ras gene protein were analyzed with flow cytometry. Results: After treating with AC960,the inhibitory rate of3 H -TdR was 33.4% for 48h. There were tipical apoptotic peaks in DNA histigram,the apoptotic rate was 21 .25% for 24h,27.77% for 48h,AC960 arrested cell cycling at S- phrase at early and at G0/G1 phrase at later stage .Expression of p53 enhanced,but bal-2 and ras reduced. Conclusion:The inhibition of proliferation,induction of apooptosis via effect on the expression of onco- gene and tumor suppressor gene might be the man anti-hepatoma action mechanism of AC960.
出处
《中国中医药科技》
CAS
2000年第1期10-12,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
上海市教委科技发展基金
上海市博士点建设基金资助项目
