摘要
目的 研究大鼠肝脏缺血性损伤后肝组织的病理学改变,以及柴胡煎剂的治疗作用。 方法 建立大鼠肝门完全阻断的模型,观察肝脏缺血性损伤后柴胡组和普食组在不同的时间阶段光镜和电镜下肝组织的病理学改变。 结果 电镜观察发现,术后1d各组肝窦内皮细胞孔隙加大,内皮破坏,内皮之间可见孔道,内皮细胞核内染色质浓聚,核膜仍完整;肝细胞肿胀,线粒体明显肿胀,基质变化,粗面内质网肿胀,脱颗粒,粗面内质网增多,细胞内糖原减少,细胞内溶酶体增多。细胞膜特化部分如桥粒、毛细胆管区微绒毛有轻微破坏。术后4d普食组肝窦内皮细胞孔隙大,内皮细胞核内染色质浓集于核膜;肝细胞肿胀,线粒体肿胀,基质密度变淡,内嵴结构模糊;粗面内质网排列不规则,脱颗粒,细胞膜破裂,肿胀变性的细胞器游离于胞外。柴胡组肝细胞轻度肿胀,核形态规则,出现吞饮小泡,肝细胞线粒体轻度肿胀,结构模糊,部分粗面内质网轻度扩张,糖原颗粒减少,可见溶酶体及脂滴,内皮膜欠光滑,基底部与肝细胞膜联系欠紧密。术后7d普食组肝细胞仍见轻度肿胀,线粒体轻度肿胀,结构模糊,基质变化,其内嵴变短,膜结构欠清楚;粗面内质网排列欠规则,糖原颗粒较少,可见少量溶酶体及脂滴。内膜欠完整,基底部与肝细胞联系欠紧密,柴胡组肝细胞未见肿胀,基本恢复到正常形态与结构,?
AIM To investigate the pathological changes of liver tissue after acute ischemic injury and the effect of bupleurum polyclonum on such damage in rats. METHODS Rat hepatic portal blockage model was established. The pathologic changes were observed dynamically by light microscopy and electron microscopy after the liver ischemic injury in bupleurum polyclonum feeding group (BPG, n = 24) and generally feeding group (GFG, n = 24).RESULTS On the first day after operation, the rat s hepatocytes showed severe retrograde degeneration, and the changes of endothelial cells were found with enlargement of the foramen between endothelial cells within the liver sinusoid and destruction in endothelial layers. On the 4th day after operation, granule changes and glycogen granulocyte reduction and chromatolysis were found in mitochondria of hepatocytes of the generally feeding group. But during this period, the specific structure of hepatocyte membrana such as desmosome and microvilli within the cholangiole could be viewed. On the 7th day after operation, the hepatocytes remained almost normal in the bupleurum polyclonum feeding group, and the changes of mitochondrion and rough neoplasmic reticulum were severe in the generally feeding group, including declination of mitochondrial matrix.CONCLUSION Bupleurum polyclonum can promote hepatocyte regeneration, restore liver injury. This result confirms the effect of bupleurum polyclonum on liver ischemic injury and establishes the pathological foundation for application of bupleurum polyclonum during perioperation.
出处
《世界华人消化杂志》
CAS
2000年第2期159-161,共3页
World Chinese Journal of Digestology
关键词
中医药疗法
肝缺血
病理学
柴胡前剂
Bupleurum polyclonum /therapeutic use
liver ischemic/TDM therapy
liver ischemic/pathology
