摘要
以蕨菜[Pteridium aquilinum(L.)kuhn]幼嫩根状茎为外植体,在愈伤组织诱导和增殖培养基中设置不同的6-BA和IBA浓度,研究6-BA和IBA对蕨菜愈伤组织诱导及增殖的影响。结果表明,1/2MS+0.2mg/L 6-BA+0.4 mg/L IBA为愈伤组织的最佳诱导培养基,诱导率为82%;1/2MS+0.2 mg/L IBA+0.2mg/L 6-BA为愈伤组织的最佳增殖培养基,增殖倍数为8.07。该研究结果为6-BA和IBA在蕨菜愈伤组织培养中的合理使用提供了参考。
Using tender rhizomes of fern as explants,different concentration of 6-BA and IBA were added in callus induction and propagation mediums in order to study their effects on callus induction and propagation.The results indicated that 1/2 MS＋0.2 mg/L 6-BA＋0.4 mg/L IBA was the best callus induction medium with an induction rate of 82%;1/2 MS＋0.2 mg/L IBA＋0.2 mg/L 6-BA was the best callus proliferation medium as the callus was proliferated by 8.07 times.The results of this study could provide references for reasonable use of 6-BA and IBA in callus culture of Pteridium aquilinum.
出处
《湖北农业科学》
北大核心
2012年第2期406-408,425,共4页
Hubei Agricultural Sciences
基金
广西教育厅科研项目(200807MS012)
