摘要
目的 探讨中暑病人热应激蛋白 (HSP70 )及其抗体水平变化和其在中暑发生中的可能作用。方法 应用Westernblot法 ,测定血浆HSP70水平 ;应用酶联免疫反应技术测定血浆HSP70抗体滴度。结果 HSP70在高温中暑组为 42 11.2± 12 86 .2 (积分光密度 )、重症中暑组为 4137.8±12 0 7.5 ,与对照组 (6 0 43.5± 135 4.8)比较 ,差异均有显著性 (P <0 .0 5 )。中暑病人HSP70抗体水平在 1∶10、1∶2 0、1∶40阳性率分别为 5 4.1%、48.9%、2 1.6 % ,显著高于对照组 (2 6 .7%、13.8%、0 % ) ,差异有显著性 (P <0 .0 5 )。结论 HSP70水平的下降和HSP70抗体的出现在中暑发生过程中具有一定的作用。
Objective To explore the level of HSP70 and HSP70 antibody in heat stroke patients and their possible roles in heat stroke. Methods HSP70 level was determined by Western dot blotting,and HSP70 Ab dilution was measured by enzyme immunosorbent assay. Results The level of HSP70 was 4 211.2± 1 286.2(AS) in the heat illness group,4 137.8±1 207.5(AS) in the severe heat illness group,and 6 043.5±1 354.8(AS) in the control group.Compared with the control,the level of HSP70 decreased significantly in heat illness group and severe heat illness group( P <0.05).The HSP70 Ab positive rates in 1∶10,1∶20,1∶40 were 54.1%,48.9% and 21.6% respectively in heat illness group,but in the control group,they were 26.7%,13.8% and 0%.There were significant difference between these two groups ( P <0.05). Conclusion The decrease of the HSP70 level and the appearance of HSP70 antibody might play important role in the process of heat illness.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2000年第1期36-38,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
