摘要
背景与目的:探讨由人精子带入受精卵中的HBs和HBc基因能否在早期胚胎细胞中进行蛋白表达。材料与方法:人精子经重组质粒pIRES2-EGFP-HBV转染后,与金黄地鼠去透明带卵母细胞离体受精,选择带绿色荧光的2-细胞胚,用免疫荧光技术检测HBs和HBc基因在胚胎细胞中的蛋白表达,用ELISA方法分别对HBsAg和HBcAg进行半定量和定性分析。结果:在带绿色荧光的2-细胞胚中免疫荧光检测可见清楚的HBsAg和HBcAg阳性信号;ELISA结果表明,单个2-细胞胚内HBsAg的量小于0.064ng/ml,对HBcAg的检测得到阳性结果。结论:人精子携带到卵内的HBV基因可在早期胚胎细胞中表达表面抗原和核心抗原。
BACKGROUND AND AIM： To study the protein expression of HBs and HBc genes brought into embryos via human spermatozoa. MATERIALS AND METHODS： Human spermatozoa were transfected with recombinant plasmid pIRES-EGFP-HBV and were then fertilized with zona pellucida-free golden hamster ova. The 2-cell embryos with and without green fluorescence were collected by immunofluorescence assay and ELISA analysis, respectively, RESULTS： The immunofluorescence assay showed that no HBsAg- and HBcAg- positive signal was observed in 2-cell embryos without green fluorescence and the clear HBsAg- and HBcAg- positive signals were detected in ones with green fluorescence. ELISA analysis showed that the amount of HBsAg in a single 2-cell embryo was about 0. 064 ng/ml and the positive result for HBcAg was detected. CONCLUSION： Human sperm-mediated HBs and HBc genes are able to express their proteins in early embryonic cells.
出处
《癌变·畸变·突变》
CAS
CSCD
2008年第3期185-188,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助(39970374)
关键词
HBV基因
人精子
胚胎细胞
蛋白表达
HBV gene
human spermatozoa
embryonic cells
protein expression
