摘要
中枢神经递质在针刺镇痛中有重要的作用,近几年来的一些研究表明,中脑中缝核和导水管壁含有丰富的5-羟色胺,因此,探讨它在针刺镇痛中的变化,可为针麻作用原理的阐明提供科学的依据。考虑到微观放射自显影技术能够把组织的形态和代谢定位的动态过程统一起来进行观察。
The role played by central 5-hydroxytryptamine(5-HT) in electro-acupuncture analgesia has been studied in rats by means of autoradiography with isotopic tracers ~3H-5-HT. The purpose of the present study is to determine the localization of ~3H-5-HT in the midbrain raphe nuclei and in the mesencephalon aquaeduct as well as periaquaeductal gray matter. Parallel experiments were studied by freezing microautoradiographic method and histo-fixative microautoradiographic method. The analgesia effect of acupuncture can be enhanced or lowered by the increment or the decrement of the 5-HT level in the mibdrain raphe nuclei and in the mesencephalon aquaeduct as well as periaquaeductal gray matter. The results of the present experiments show that, when the rats were subjectad to electro-acupuncture analgesia. The miroautoradiographic intensities of ~3H-5-HT both in the midbrain raphe nuclei and in the areas of mesencephalon aquaeduct were significantly increased. It may be observed that the release of 5-HT in these regions of the brain is accelerated during acupuncture analgesia. From this it can be concluded that the midbrain raphe nuclei and the mesencephalon aquaeduct as well as the periaquaeductal gray matter are closely related to acupuncture analgesia. The results imply that 5-HT in these areas may be one of the most important neurochemical agents mediating acupuncture analgesia.
出处
《针刺研究》
CAS
CSCD
1990年第4期269-273,共5页
Acupuncture Research
