摘要
目的探讨小鼠异位小肠移植的技术要点并进行相应改进,为小肠移植的研究提供可靠的动物模型。方法 53只Balb/C小鼠的节段小肠异位移植于C57BL/6小鼠腹腔。适当缩短切取小肠及血管蒂门静脉的长度;应用缝针法建立受体腹主动脉前壁的椭圆形切口,穿刺法建立下腔静脉前壁切口;两点定位连续缝合法吻合血管;近端结扎封闭,远端与受体空肠端侧吻合重建肠道。结果 53例异位小肠移植的小鼠成活7天以上46例,成活率86.8%。供体手术时间45±10分钟,移植小肠冷缺血时间25±5分钟,受体手术时间56±7分钟,其中腹主动脉下腔静脉阻断时间33±8分钟。主要失败原因为吻合口出血过多1例,吻合时间较长致下肢瘫痪3例,肠梗阻功能紊乱1例,吻合口狭窄1例,麻醉意外1例。结论对小鼠异位小肠移植的技术要点进行改进,有利于建立稳定的模型用于科学研究。
Objective To explore and improve the skill points of heterotopic small bowel transplantation（SBT） models in mice,and construct the stable model for small bowel transplantation research.Methods 53 segmental small bowels of Balb/C mice were transplanted into abdominal cavities of C57BL/6 mice.Several skill points were improved： to abridge length of the transplanted small bowel and portal vein pedicle;to make elliptical incision on front wall of the donor ventral aorta with suture needle puncture way and form the anterior wall incision of inferior caval vein with syringe needle puncture way;to anastomose the vessels with continuous sutures;to reconstruct the intestinal tract through ligating proximal end of graft intestine and end-to-side anastomosis of distal end of graft intestine with recipient jejunum.Results 46 mice survived 7days in 53 heterotopic SBT cases.The successful rate was 86.8%.In experiments harvesting time of donors was 45±10 mins.and the graft cool ischemic time was 25±5 mins,recipient operation time was 56±7 mins,in which the abdominal aorta and IVC blockage time was 33±8 mins.The operations failed in 7cases,due to massive hemorrhage（1 case）,long anastomose time and crural paralysis（3 cases）,intestinal obstruction（1 case）,anastomosis stenosis（1case）,and anesthetic accident（1 case）.Conclusions Improvement of the skill points is facilitated establishment of stable SBT model for science research.
出处
《肝胆外科杂志》
2011年第6期458-460,共3页
Journal of Hepatobiliary Surgery
基金
国家留学基金委"建设高水平大学公派留学生项目"资助
编号:2008638052
关键词
小鼠
异位
小肠/移植
技术改进
mouse
heterotopic
small bowel/transplantation
technical improvement
