摘要
研究了不同浓度(0.01、0.05、0.10、0.20和0.30 m g/L)的水体汞(Hg2+)在不同暴露时间(1、7、14和30 d),对中华绒螯蟹(E rioche ir s inens is)(17.36±1.23)g肝胰腺超氧化物歧化酶SOD、谷胱甘肽过氧化物酶GPX和过氧化氢酶CAT活力以及脂质过氧化产物丙二醛M DA含量的影响.结果表明,较低浓度的Hg2+(0.01和0.05 m g/L)处理组中华绒螯蟹肝胰腺超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力在短时间内上升,表现出一种"毒物兴奋效应";随着暴露时间的延长,SOD和CAT酶活力均受到明显抑制;高浓度的汞(0.20和0.30m g/L)无论暴露时间长短,对3种抗氧化酶均产生明显抑制作用.不同浓度的Hg2+处理组中华绒螯蟹肝胰腺M DA含量均高于对照组,表现出"剂量-效应"和"时间-效应"关系.双因素方差分析结果表明,水体Hg2+的浓度、暴露时间以及二者之间的相互作用均对中华绒螯蟹肝胰腺3种抗氧化酶和M DA含量产生显著影响.肝胰腺SOD、GPX、CAT活力以及M DA含量对水环境中的Hg2+反应灵敏,可作为中华绒螯蟹毒理学评价指标以及重金属污染监测指标.
To explore the effects of mercury（Hg2＋） exposure on the antioxidant enzyme activities and the level of lipid peroxidation of the hepatopancreas,Chinese mitten crabs Eriocheir sinensis were exposed to Hg2＋（0.01,0.05,0.10,0.20,0.30 mg/L） for 1,7,14,30 d.The results showed that the activities of superoxide dismutase（SOD）,glutathione peroxidase（GPX） and catalase（CAT） in the groups treated with 0.01 and 0.05 mg/L Hg2＋ in a short time（1 and 7 d） was significantly increased,which appeared a ＂Poison-intoxication effect＂;however,these antioxidant enzyme activities decreased with increasing exposure time.In contrast,enzyme activities in the presence of 0.20 and 0.30 mg/L Hg2＋ was decreased no matter how long the exposure time was.The level of lipid peroxidation weighted as MDA content in hepatopancreas of crab exposed to different Hg2＋ concentrations were significantly higher than those of controls,which appeared ＂dose-effect＂ and ＂time-effect＂.The results of two ways analysis of variance showed that both Hg2＋ concentration and exposure time had significant effects on these three antioxidant enzyme activities and MDA content.According to present study,SOD,GPX and CAT activities and MDA content in hepatopancreas of E.sinensis were sensitive to ambient Hg2＋ and could be used as biomarkers in evaluating the toxicity of mercury or the other heavy metals contamination to crustacean.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第5期88-95,共8页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
国家水体污染控制与治理科技重大专项资助项目(2009ZX07528-002)
