摘要
为给海湾扇贝的细胞免疫学研究提供基本依据,同时采用光镜和电镜技术观察海湾扇贝血细胞的形态特征,并进行血细胞酸性磷酸酶活性的初步分析。结果表明,海湾扇贝的血细胞可分为透明细胞和颗粒细胞2种类型。与透明细胞相比,颗粒细胞数目多,酸性磷酸酶活性高,细胞核呈肾形或卵圆形,核质比低,细胞质内颗粒状物质丰富,颗粒大小不一,可明显分为大颗粒细胞和小颗粒细胞2类。这些特征表明,海湾扇贝颗粒细胞和透明细胞的免疫学功能是明显不同的。
Two types of haemocytes were identifed from the Bay scallop (Argopecten irradians) by means of Giemsa stain. Hyalinocytes have a large round nucleus with a higher nucleocytoplasmic (N/C) ratio surrounded by a thin cytoplasmic layer; some hyalinocytes have double nucleus. Granulocytes are more abundant and larger than hyalinocytes but their N/C ratio is lower. Under the TEM, compared with hyalinocytes, granulocytes possess relatively more granules and organelles in their cytoplasm. Stainness by acid phosphatase (ACP) indicated that ACP in both types of haemocytes could be detected and the ACP activity of granulocytes was higher than that of hyalinocytes.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期404-406,共3页
Periodical of Ocean University of China
基金
国家重点基础研究发展规划项目计划 (G1 9990 1 2 0 0 1 )资助
关键词
海湾扇贝
血细胞
酸性磷酸酶
超微结构
Argopecten irradians
haemocyte
the acid phosphatase (ACP)
ultrastructure
