摘要
背景:胰蛋白酶已被证实具有较好的清创、促进创伤愈合作用。目的:探讨南极大磷虾胰蛋白酶对创面的修复效果。方法:采用全层皮肤缺损法制成新西兰大白兔背部创伤模型,将创面分为4组,分别给予从南极大磷虾中提取的粗酶液或纯化的胰蛋白酶、木瓜蛋白酶和生理盐水,每天早晚两次给药,直至伤口愈合。结果与结论:给药5,10和15d测量伤口愈合面积,从南极大磷虾中提取的粗酶和纯化胰蛋白酶均具有良好的促进愈合效果,但粗酶的效果更好,其各时段残余创面面积小于生理盐水组和木瓜蛋白酶组(P<0.05);皮肤羟脯氨酸含量测定结果显示粗酶能显著促进创伤组织中胶原的合成和积累;组织学切片观察显示粗酶组愈合效果最佳。证明南极大磷虾胰蛋白酶能显著促进新西兰大白兔创口的愈合,粗酶具有最优效果。
BACKGROUND： It is confirmed that trypsin has better effects on debridement and wound healing. OBJECTIVE： To explore the effects of the Antarctic krill trypsin on wound healing. METHODS： Back trauma model in New Zealand white rabbits was established by defecting full-thickness skin. There were four wounds in each rabbit, treated with crude trypsin, pure trypsin extracted from Antarctic krill, papin and saline, respectively. The wounds were treated twice daily until healed. RESULTS AND CONCLUSION： According to the healing area at the 5th, 10th and 15Ih days after treatment, crude trypsin and pure trypsin extracted from Antarctic krill had a better effect on wound healing. Crude trypsin was better than pure trypsin. The residual wounds area of crude typsin group for each time period was smaller than that of saline group and papain group （P 〈 0.05）. The content determination results of skin hydroxyproline showed that crude trypsin significantly improved the synthesis and accumulation of collagen in wound tissue. According to histological sections, crude typsin group achieved the best efficiency of the treatment. These results demonstrate that Antarctic krill trypsin can promote wound healing in New Zealand white rabbits, and the crude typsin has the best effect.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第50期9449-9452,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
山东省自然科学基金(ZR2010DQ012)资助项目
项目名称:南极大磷虾(Euphausia superba)胰蛋白酶适冷性结构特征及酶学性质研究~~
