摘要
目的:建立测定肠腹宁颗粒中白头翁皂苷B4含量的方法。方法:色谱柱为Hypersil C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(30∶70),流速为1.0 mL·min-1,柱温为室温,检测波长为205 nm,进样量为10μL。结果:白头翁皂苷B4的质量浓度在3.125～50.000μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.999 8);平均加样回收率为99.45%,RSD=2.83%(n=6)。结论:该方法专属性强、灵敏度高、重复性好,可用于肠腹宁颗粒的质量控制。
OBJECTIVE： To establish a method for the content determination of pulchinenoside B4 in Changfuning granules, METHODS： The determination was performed on the Hypersil C18（250 mm×4.6 mm, 5 μm） column at room temperature. The mobile phase was consisted of acetonitrile-0.1%phosphoric acid （30：70） at the flow rate of 1.0 mL. min^-1 and detection wavelength of 205 nm. The injection volume was 10μL. RESULTS： The linear range of pulchinenoside B4 was 3.125-50.000 μg. mL^-1（r= 0.999 8）with an average recovery of 99.45% （RSD=2.83% ,n=6）. CONCLUSION：The method is specific, sensitive and reproducibility for the quality control of Changfuning granules.
出处
《中国药房》
CAS
CSCD
2012年第3期246-247,共2页
China Pharmacy
基金
广东省中山市卫生局科研项目(2009079)
